Development of Dispersive Liquid-Liquid Microextraction Procedure for Trace Determination of Malathion Pesticide in Urine Samples.

Background: Measurement of pesticides in biological matrices is become a serious challenge for researches because of their very low concentration in different matrices. The aim of this study was to develop a new sample preparation method with high accuracy and validity, simplicity and short retention time for determination of malathion.

Methods: Dispersive liquid-liquid micro-extraction (DLLME) technique coupled with high-performance liquid chromatography equipped with ultraviolet detector (HPLC-UV) developed for trace extraction and determination of malathion pesticide in human urine samples.

Optimization of dispersive liquid-liquid microextraction procedure for detecting chlorpyrifos in human urine samples.

Selecting an effective sample preparation method to measure target pesticides in biological matrices is a serious challenge for researchers. This study aimed to optimize the dispersive liquid-liquid microextraction (DLLME) technique to obtain a simple, valid, and fast method with high efficiency to detect chlorpyrifos in urine samples. DLLME, coupled with high performance liquid chromatography equipped with ultra violet detector, was used to extract chlorpyrifos pesticide in human urine samples.