An model system based on calcium- and phosphate ion-induced hMSC spheroid mineralization.

A challenge in regenerative medicine is creating the three-dimensional organic and inorganic microenvironment of bone, which would allow the study of musculoskeletal disorders and the generation of building blocks for bone regeneration. This study presents a microwell-based platform for creating spheroids of human mesenchymal stromal cells, which are then mineralized using ionic calcium and phosphate supplementation. The resulting mineralized spheroids promote an osteogenic gene expression profile through the influence of the spheroids' biophysical environment and inorganic signaling and require less calcium or phosphate to achieve mineralization compared to a monolayer culture.

Physical, mechanical and biological performance of PHB-Chitosan/MWCNTs nanocomposite coating deposited on bioglass based scaffold: Potential application in bone tissue engineering.

Nowadays, using the nanocomposite coatings on bioceramic scaffolds is a great interest for many researchers to improve the properties of these scaffolds. In this study, the effect of poly (3-hydroxybutyrate) PHB-Chitosan (Cs)/multi-walled carbon nanotubes (MWCNTs) nanocomposite coating deposited on nano-bioglass (nBG)-titania (nTiO) scaffolds fabricated by foam replication method was investigated. Structural analyses such as XRD and FT-IR confirmed the presence of PHB, Cs and MWCNTs in the coated scaffolds.