Optimizing cancer treatment: the synergistic potential of CAR-T cell therapy and CRISPR/Cas9.

Optimizing cancer treatment has become a pivotal goal in modern oncology, with advancements in immunotherapy and genetic engineering offering promising avenues. CAR-T cell therapy, a revolutionary approach that harnesses the body's own immune cells to target and destroy cancer cells, has shown remarkable success, particularly in treating acute lymphoblastic leukemia (ALL), and in treating other hematologic malignancies. While CAR-T cell therapy has shown promise, challenges such as high cost and manufacturing complexity remain.

Optimizing Nutritional Decisions: A Particle Swarm Optimization-Simulated Annealing-Enhanced Analytic Hierarchy Process Approach for Personalized Meal Planning.

Background/objective: Nutritionists play a crucial role in guiding individuals toward healthier lifestyles through personalized meal planning; however, this task involves navigating a complex web of factors, including health conditions, dietary restrictions, cultural preferences, and socioeconomic constraints. The Analytic Hierarchy Process (AHP) offers a valuable framework for structuring these multi-faceted decisions but inconsistencies can hinder its effectiveness in pairwise comparisons.

Methods: This paper proposes a novel hybrid Particle Swarm Optimization-Simulated Annealing (PSO-SA) algorithm to refine inconsistent AHP weight matrices, ensuring a consistent and accurate representation of the nutritionist's expertise and client preferences.

Enhancing shear strength predictions of rocks using a hierarchical ensemble model.

Shear strength (SS) parameters are essential for understanding the mechanical behavior of materials, particularly in geotechnical engineering and rock mechanics. This study proposes a novel hierarchical ensemble model (HEM) to predict SS parameters: cohesion ( ) and angle of internal friction ( ). The HEM addresses the limitations of traditional machine learning models.

Advances and challenges in gene therapy strategies for pediatric cancer: a comprehensive update.

Pediatric cancers represent a tragic but also promising area for gene therapy. Although conventional treatments have improved survival rates, there is still a need for targeted and less toxic interventions. This article critically analyzes recent advances in gene therapy for pediatric malignancies and discusses the challenges that remain.

Photodynamic Therapy in a Lateral Maxillary Incisor with Dens in Dente Type Ⅱ and Primary Endodontic Lesion: Case Report.

Endodontic treatment in dens invaginatus anomaly is associated with challenges in all stages. This case report outlines the therapy provided for tooth #10 with occasional pain. In examinations, tenderness to percussion and touch and non-response to sensibility tests were observed, and pulp necrosis and symptomatic periapical periodontitis were diagnosed.

Microfluidic System Consisting of a Magnetic 3D-Printed Microchannel Filter for Isolation and Enrichment of Circulating Tumor Cells Targeted by Anti-HER2/MOF@Ferrite Core-Shell Nanostructures: A Theranostic CTC Dialysis System.

Low number of circulating tumor cells (CTCs) in the blood samples and time-consuming properties of the current CTC isolation methods for processing a small volume of blood are the biggest obstacles to CTC usage in practice. Therefore, we aimed to design a CTC dialysis system with the ability to process cancer patients' whole blood within a reasonable time. Two strategies were employed for developing this dialysis setup, including (i) synthesizing novel core-shell Cu ferrites consisting of the Cu-CuFeO core and the MIL-88A shell, which are targeted by the anti-HER2 antibody for the efficient targeting and trapping of CTCs; and (ii) fabricating a microfluidic system containing a three-dimensional (3D)-printed microchannel filter composed of a polycaprolactone/FeO nanoparticle composite with pore diameter less than 200 μm on which a high-voltage magnetic field is focused to enrich and isolate the magnetic nanoparticle-targeted CTCs from a large volume of blood.

Shut-up Toys for Second Language Learners: Impact of Digital Media on Early Adolescents' Private Speech Production in Individual and Collaborative Tasks.

Researchers, parents, and policymakers from previous generations have recently expressed concern about the inevitable exposure of youngsters to digital media and its potentially detrimental effects on their development. Private speech is the overt audible self-talk people produce when engaged with challenging problem-solving tasks and is believed to aid in second language acquisition as reported (Vygotsky in Thought and language, MIT Press, 1962); (Winsler in Private Speech, Executive Functioning, and the Development of Verbal Self-Regulation, 2009). This qualitative case study explored private speech production in three young adolescents (two 11-year-olds and one 10-year-old) while completing an English as a foreign language task (Bingo! game) individually and collaboratively in physical and digital modes.

Delighting Palates with AI: Reinforcement Learning's Triumph in Crafting Personalized Meal Plans with High User Acceptance.

Eating, central to human existence, is influenced by a myriad of factors, including nutrition, health, personal taste, cultural background, and flavor preferences. The challenge of devising personalized meal plans that effectively encompass these dimensions is formidable. A crucial shortfall in many existing meal-planning systems is poor user adherence, often stemming from a disconnect between the plan and the user's lifestyle, preferences, or unseen eating patterns.

Guided Endodontic Treatment for Calcified Central Incisor with Discoloration, a Conservative Approach Preserving Incisal Edge: A Case Report.

Pulp canal obliteration (PCO) in calcified teeth presents a challenge for endodontic treatment, especially when accompanied by discoloration. Guided endodontic access (GEA) has emerged as an advanced method for root canal therapy (RCT) in such cases. This case report describes the successful treatment of a calcified maxillary central incisor with discoloration using GEA.

Co-expression network analysis for the identification of potential prostate cancer genes and in vitro confirmation of their expression in cell model in the presence of Staphylococcal gene.

Prostate cancer has arisen as an important life-threatening malignancy in males worldwide. Therefore, it is important to study underlying molecular pathways to be able to proposed appropriate a novel pathway of apoptosis in prostate cancer. This study aimed to explore the molecular effects of Staphylococcal tsst-1 gene on PC3 cell line apoptosis by in silico and studies.

Personalized Flexible Meal Planning for Individuals With Diet-Related Health Concerns: System Design and Feasibility Validation Study.

Background: Chronic diseases such as heart disease, stroke, diabetes, and hypertension are major global health challenges. Healthy eating can help people with chronic diseases manage their condition and prevent complications. However, making healthy meal plans is not easy, as it requires the consideration of various factors such as health concerns, nutritional requirements, tastes, economic status, and time limits.

Treatment of Peri-Invagination Lesion and Vitality Preservation in Type III Dens Invaginatus in Bilateral Immature Maxillary Lateral Incisors: A Case Report.

The superior lateral incisors are primarily affected by the developmental deformity known as dens invaginatus (DI). Oehler's type III DI has the highest complexity rendering a root canal treatment (RCT) an arduous challenge for this type, so early diagnosis and treatment before pulp involvement are important. This report presents two maxillary lateral incisors with type IIIb DI, the left one being associated with a periapical lesion and the right one with normal pulp.

The Evaluation of Debris and Smear Layer Generated by Three Rotary Instruments Neo NiTi, 2Shape and Revo_S: An Scanning Electron Microscopic Study.

Introduction: This study compared the cleaning effectiveness of NeoNiTi, 2Shape and Revo_S rotary instruments.

Materials And Methods: Fifty mandibular molar mesial roots were selected with an angle of curvature less than 20 degrees divided into three groups (=15). Five samples were selected as negative control group.

Impact of ketamine administration on chronic unpredictable stress-induced rat model of depression during extremely low-frequency electromagnetic field exposure: Behavioral, histological and molecular study.

Objectives: In the study, we examined the effects of ketamine and extremely low-frequency electromagnetic fields (ELF-EMF) on depression-like behavior, learning and memory, expression of GFAP, caspase-3, p53, BDNF, and NMDA receptor in animals subjected to chronic unpredictable stress (CUS).

Methods: After applying 21 days of chronic unpredictable stress, male rats received intraperitoneal (IP) of ketamine (5 mg/kg) and then were exposed to ELF-EMF (10-Hz, 10-mT exposure conditions) for 3 days (3 h per day) and behavioral assessments were performed 24 h after the treatments. Instantly after the last behavioral test, the brain was extracted for Nissl staining, immunohistochemistry, and real-time PCR analyses.

Fabrication of nanosheet-assembled hollow copper-nickel phosphide spheres embedded in reduced graphene oxide texture for hybrid supercapacitors.

Owing to their metalloid characteristics with high electrical conductivity, transition metal phosphides (TMPs) have attracted considerable research attention as prospective cathodes for hybrid supercapacitors. Unfortunately, they usually exhibit low rate performance as well as poor longevity, which does not meet the demands of hybrid supercapacitors. The nanocomposite constructed from reduced graphene oxide (rGO) and TMPs with a highly porous nature can effectively overcome the above-mentioned issues, greatly widening their utilization.

Cobalt-molybdenum selenide double-shelled hollow nanocages derived from metal-organic frameworks as high performance electrodes for hybrid supercapacitor.

In this paper, we developed a sequential chemical etching and selenization processes to synthesize Co-MoSex double-shelled hollow nanocages (CMS-DSHNCs) as high performance electrode materials for supercapacitor applications. Co-MoO yolk-shelled hollow nanocages were firstly synthesized using a solvothermal process through facile ion-exchange reactions between zeolitic imidazolate framework-67 (ZIF-67) and MoO ions. By applying a solvothermal temperature of 160 °C in the presence of SeO and subsequently annealing strategy, CMS-DSHNCs were successfully synthesized with a yolk-shell hierarchically hollow and porous morphology of mixed metal selenides.

A dual surface inorganic molecularly imprinted BiWO-CuO/AgO heterostructure with enhanced activity-selectivity towards the photocatalytic degradation of target contaminantst.

In this work, a high-surface-area dual inorganic molecularly imprinted (DIMI) BiWO/CuO/AgO photo-catalyst was developed for the selective photocatalytic degradation of methyl green (MG) and auramine O (AO) dyes as target pollutants. The DIMI-BiWO/CuO/AgO heterojunction was synthesized by a sono-chemically assisted sol-gel method by coating a layer of molecularly imprinted AgO/CuO on the surface of BiWO nanocubes with MG and AO as the templates. This was followed by calcination for the removal of target molecules and annealing for Ag/Cu oxide preparation.

Lactoferrin suppresses LPS-induced expression of HMGB1, microRNA 155, 146, and TLR4/MyD88/NF-кB pathway in RAW264.7 cells.

Objective: This current study evaluated the underlying mechanisms of LF against the inflammatory microRNAs (miRNAs), HMGB1 expression, and TLR4-MyD88-NF-кB pathway in LPS-activated murine RAW264.7 cells.

Methods: MTT assay was used to assess cell metabolism and the cell culture levels of the cytokines (TNF-α, IL-6) were evaluated by Enzyme-linked immunosorbent assay (ELISA).

Inhibitory effect of melatonin on hypoxia-induced vasculogenic mimicry via suppressing epithelial-mesenchymal transition (EMT) in breast cancer stem cells.

Vasculogenic mimicry (VM) play an important role in breast cancer metastasis and anti- angiogenic drugs resistance. Hypoxia, the epithelial-mesenchymal transition (EMT), and cancer stem cells (CSCs) are known as essential factors for VM formation. Also, melatonin is an amino acid-derived hormone with many anti-tumor effects.

A BiWO/AgS/ZnS -scheme heterojunction photocatalyst with enhanced visible-light photoactivity towards the degradation of multiple dye pollutants.

A novel visible-light-driven -scheme heterojunction, BiWO/AgS/ZnS, was synthesized and its photocatalytic activity was evaluated for the treatment of a binary mixture of dyes, and its physicochemical properties were characterized using FT-IR, XRD, DRS and FE-SEM techniques. The BiWO/AgS/ZnS -scheme heterojunctions not only facilitate the charge separation and transfer, but also maintain the redox ability of their components. The superior photocatalytic activity demonstrated by the -scheme BiWO/AgS/ZnS attributes its unique properties such as the rapid generation of electron-hole pairs, slow recombination rate, and narrow bandgap.

The effect of nurse empowerment educational program on patient safety culture: a randomized controlled trial.

Background: The complexity of patients' condition and treatment processes in intensive care units (ICUs) predisposes patients to more hazardous events. Effective patient safety culture is related to lowering the rate of patients' complications and fewer adverse events. The present study aimed to determine the effect of empowering nurses and supervisors through an educational program on patient safety culture in adult ICUs.

Cytotoxicity of carboplatin on human glioblastoma cells is reduced by the concomitant exposure to an extremely low-frequency electromagnetic field (50 Hz, 70 G).

Glioblastoma multiforme (GBM) is a malignant brain cancer that causes high mortality in patients. GBM responds weakly to the common cancer treatments such as chemotherapy and radiotherapy and even surgery. Carboplatin is an alkylating agent widely used to treat cancer.

Cytotoxic Effects of the Ethanol Bane Skin Extract in Human Prostate Cancer Pc3 Cells.

Background: It is extensively supposed that vegetarian diet could affect cancer progress and increase the influence of formal chemotherapy.

Objectives: The present study was designed to determine the effect of the ethanol Bane skin extract against chemo resistant prostate cancer PC3 cells.

Materials And Methods: PC3 and L929 cells were cultivated and then incubated in the ethanol Bane skin extract with various concentrations of 0.

Comparison of Cytotoxic Activity of Bile on HepG2 and CCRF-CEM Cell Lines: An in Vitro Study.

The aim of this study was to examine the effect of crude bile on the human HepG2 and CCRF-CEM cell lines. Cells were exposed to different dilutions of bile. Antiproliferative effects were determined by the cytotoxic MTT assay.