Publications by authors named "Mary Canzano"
Excitation-Dependent pKa Extends the Sensing Range of Fluorescence Lifetime pH Sensors.
Sensors (Basel)
November 2024
Article Synopsis
- Biological activity is influenced by pH levels, which vary across different environments, prompting the need for specialized sensors.
- A variety of fluorescent sensors have been developed to measure pH in live cells, with lifetime-based sensors offering valuable quantification advantages.
- This research shows how the apparent pKa of these sensors is affected by the wavelength of light used for excitation, enabling a single sensor to effectively detect pH in multiple physiological contexts.
Targeted drug delivery approaches that selectively and preferentially deliver therapeutic agents to specific tissues are of great interest for safer and more effective pharmaceutical treatments. We investigated whether cathepsin B cleavage of a valine-citrulline [VC()]-containing linker is required for the release of monomethyl auristatin E (MMAE) from albumin-drug conjugates. In this study, we used an engineered version of human serum albumin, Veltis High Binder II (HBII), which has enhanced binding to the neonatal Fc (fragment crystallizable) receptor (FcRn) to improve drug release upon binding and FcRn-mediated recycling.
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