Single-cell measurement of higher-order 3D genome organization with scSPRITE.

Although three-dimensional (3D) genome organization is central to many aspects of nuclear function, it has been difficult to measure at the single-cell level. To address this, we developed 'single-cell split-pool recognition of interactions by tag extension' (scSPRITE). scSPRITE uses split-and-pool barcoding to tag DNA fragments in the same nucleus and their 3D spatial arrangement.

How can chemometrics improve microfluidic research?

Chemometrics has the potential to embolden microfluidics to become that enabling technology for so long sought after. In this Feature article, we describe a historical perspective on microfluidics and its current challenges, a perspective on chemometric methods including response surface methodology (RSM), and how a combination of artificial neural network with experimental design (ANN-ED) have demonstrated promise in addressing basic microfluidic problems.

Development of a microfluidic-based assay on a novel nitrocellulose platform.

A novel microfluidic paper-based analytical device (μPAD) utilizing a nitrocellulose (NC) membrane to detect IgG antibodies through a colorimetric analysis is described. The μPAD was constructed using layered polyethylene terephthalate (PET) and pressure-sensitive adhesives (PSA). The biotin labeled Goat Anti-Mouse IgG antibody was spotted and dried on the NC channel prior to subjecting it to a series of wash solutions (Tris-tween), increasing concentrations of alkaline phosphatase conjugated to streptavidin (Strep-ALP), and para-nitrophenyl phosphate (p-NPP) realizing a vibrant yellow color.