A histochemical study of changes in mitochondrial enzyme activities of rat liver after ischemia in vitro.

Changes in the activity of three mitochondrial enzymes in rat liver after in vitro ischemia have been determined by enzyme histochemical methods. The changes were correlated with the appearance in the electron microscope of flocculent densities in the mitochondria indicative of irreversible cell injury. The flocculent densities were observed in rat liver after about 2 h of ischemia in vitro at 37 degrees C.

Influence of nutritional state on ischemic damage in rat liver.

The influence of the nutritional state of rats on the volume of necrosis in the liver at 24 h after 60 min ischemia has been estimated using fresh liver slices incubated in tetranitro BT. The volume of necrosis is about 30% in an animal fed ad libitum and about 10% in rats fasted for 24 h. Standardization of the time of feeding using a food dispensing apparatus did not reduce the relatively high individual variation in the extent of liver cell necrosis after ischemia.

[Ureterotransversopyelostomy with unilateral nephrostomy].

Supravesical urinary diversion by ureterotransversopyelostomy (UTPS) with unilateral nephrostomy was performed in 57 patients. The age of the 33 women ranged between 42 and 86 (mean 65), of the 24 men between 39 and 77 (mean 62) years. With a single exception, the indication for diversion was palliative: 25 patients had advanced bladder cancer (T3/T4), and 19 had undergone irradiation; 24 patients showed vesico- (recto-) vaginal fistulas due to radiation for gynecological carcinomas.

Quantitative aspects of the histochemical tetrazolium salt reaction on monoamine oxidase activity in rat liver.

The tetrazolium method for the histochemical detection of monoamine oxidase (MAO) activity in rat liver cryostat sections has been tested for its specificity and its possible use in quantification. The tetrazolium salt tetranitro blue tetrazolium is recommended for the localization of MAO activity, rather than nitro blue tetrazolium or BPST [2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium] . Hardly any formazan was produced in the absence of the substrate tryptamine and Marsilid, a specific inhibitor of MAO activity, prevented formazan production almost completely.

[Correction of idiopathic juvenile penis deviation with the modified Nesbit operation].

In seven men a congenital penile curvature was corrected using a modified Nesbit operation. All patients obtained an excellent correction of the deformity and satisfactory intercourse. In three cases a small area of hypesthetic skin was observed in the distal parts of the dorsum penis.

[The value of hypothermia methods in operative stone removal].

In spite of the possibility of using ultrasound for identification of stone fragments and vascular localisation, it is still necessary to clamp the renal vascular pedicle in selected cases. In situ perfusion achieves a nearly complete restoration of renal function, but requires a more sophisticated technique and preoperative planning. Surface cooling on the other hand is universally applicable and can be used during surgery, when unforeseen clamping of the renal pedicle is needed.

[Therapy of acute radiation cystitis. A case report].

We report a patient with prostate cancer who suffered from severe radiation cystitis after combined interstitial radiation with 125-Iodine-Seeds and external radiation (2000 rad). This patient was treated very successfully with intramural injection of Orgotein (Peroxinorm) into the bladder wall. Besides discussion of the aetiology of radiation cystitis we report our first favourable results with local and systemic treatment with Orgotein (Peroxinorm).

[Percutaneous nephrostomy as primary therapy of ureterovaginal fistula].

We report on our experience with 11 cases of ureterovaginal fistula primarily treated with percutaneous nephrostomy drainage. In 6 patients the fistula persisted and ureteral reimplantation with psoas hitch was performed. In 5 patients the ureterovaginal fistula closed during nephrostomy drainage.

[Limitations and hazards of palliative renal tumor embolization].

Today the indication for palliative embolization of inoperable renal carcinoma is more restricted than several years ago. Reviewing 31 own palliative occlusions of the renal artery in 29 patients over a period of 5 1/2 years two main reasons for this attitude are presented: 1. Because of collateral or parasitic vascular supply of kidney tumors the occlusion of the renal arteries only results in a retarded tumor growth rate and does not seem to prolong patient survival.

[Therapy of priapism (author's transl)].

Due to the poor knowledge concerning etiology and pathophysiology of priapism the treatment necessarily remains largely symptomatic. There are two main therapeutic aims: 1. The penile detumescence in order to relief the often severe local pain and 2.

[Radionuclide angiography (RNA) with 99mTc-DTPA to follow palliative embolization of renal cell carcinoma (author's transl)].

In 14 patients with renal cell carcinoma, not to be treated by nephrectomy, radionuclide angiography with 99mTc-DTPA (RNA) was employed before and after palliative tumor embolization. If RNA revealed remaining perfusion of the tumor, functional scintigraphy with 131J-hippurate was performed to detect and quantify residual function. Up to 16 months after embolization, 7 patients (50%) revealed neither residual perfusion nor function.

[Experience with transrenal ureteral occlusion (author's transl)].

Closure of the ureter with a mixture of butyl-2-cyano-acrylate and lipiodol was performed for the palliative management of urinary incontinence in 4 patients (unilaterally in 3, bilaterally in 1), after urinary diversion had been provided by operative or percutaneous nephrostomy (inoperable vesico-(recto-)vaginal fistulas [2 patients] due to irradiated cervical carcinomas, contracted bladder due to irradiation [1 patient] and irradiated locally advanced prostatic carcinoma [1 patient]). In the first two patients the ureters were occluded by transurethral access using ureteral catheters followed in one case by dislocation of embolic material in the renal pelvis (without sequelae). The transrenal access for ureteral closure was employed in the two other patients using adjuvant balloon catheter occlusion.

Studies on the clinical significance of nonesterified and total cholesterol in urine.

Gas-liquid chromatographic determinations of nonesterified and total urinary cholesterol were performed in 137 normals, 264 patients with various internal diseases without evidence of neoplasias or diseases of the kidney or urinary tract, 497 patients with malignancies and 236 patients with diseases of the kidney, urinary tract infections or prostatic adenoma with residual urine. A normal range (mean +/- 2 SD) of 0.2-2.

A new technique for percutaneous nephropyelostomy.

Primary insertion of a large caliber catheter into the renal pelvis would provide the most favorable drainage in all cases in which urinary diversion by nephropyelostomy is indicated. Therefore, the puncture technique of Guenther and associates was modified. A specially designed balloon catheter was used to prevent dislocation.

[Local treatment of urethral condylomata by 5-fluorouracil].

4 male patients suffering from urethral condylomata were treated with local 5-Fluorouracil. 3 different ways of application were used: Gel, Suppositories and Cream (5%). Treatment for 5.

[Suprapubic drainage of the bladder (author's transl)].

The principal advantages of suprapubic drainage of the bladder compared with the transurethral indwelling catheter are to be seen in holding back ascending infection of the urinary tract and avoidance of urethral trauma. In addition to relieving the bladder in subvesical obstructions the most important indication in practise is continuous drainage of urine in patients in intensive care. With strict observation of puncture technique and contraindications (contracted bladder, tumors in the lower abdomen, bladder carcinoma, macrohematuria and hemorrhagic diathesis) the entire complication rate is 4.

Cancer detection: industrial screening programs.

Development of oncologic occupational medicine has an interesting history and emphasizes the need for early detection of cancers in the industrial environment.