E-DNA detection of rpoB gene resistance in Mycobacterium tuberculosis in real samples using FeO/polypyrrole nanocomposite.

In this work, we achieved the selective detection of wild and mutated rpoB gene in M. tuberculosis using an electrochemical DNA (E-DNA) sensor based on polypyrrole/FeO nanocomposite bearing redox naphthoquinone tag on PAMAM (spaNQ/PAMAM/PPy/FeO). The hybridization between a given probe and the complementary DNA target induced a large decrease in the naphthoquinone redox signal as measured by SWV and no cross-hybridization with single nucleotide mismatch DNA target occurred.