Effect of diode pumping solid state laser with wavelength 589 nm on gene expression of interlukine-2 and interferon-γ in human T-lymphocytes.

Objective: To evaluate the impact of laser irradiation of human T-cell lymphocyte in culture to determine the photobiomodulatory effects of such irradiation on gene expression, and the release of growth factors and cytokines.

Methods: The experimental study was conducted from November 2021 to April 2022 at the Postgraduate Anatomy Laboratory, Mustansiriyah University, Baghdad, Iraq, and comprised human blood samples T-cell lymphocytes were extracted and irradiated, followed by the extraction of ribonucleic acid to assess the effect of laser on gene expression of cytokines, interlukine-2 and interferon-gamma. A 589nm yellow light low-level laser beam from a diode pumping solid state laser was used at doses 30J/cm2, 50J/cm2 and 70J/cm2 for an exposure time of 15 minutes.

The effect of a diode-pumped solid-state laser with a wavelength of 589 nm on T-lymphocyte proliferation.

Objective: To assess how low-level laser irradiation affects T cells.

Methods: The case-control interventional study was conducted from November 2021 to April 2022 after approval by the ethics review committee of the College of Medicine, Mustansiriyah University, Baghdad, Iraq, and comprised healthy individuals. Phlebotomy was used to collect 4ml of blood.