sp. nov., isolated from surface of the maize () roots in a horticulture field, Hungary.

A novel Gram-stain-positive, rod-shaped, endospore-forming bacterium with peritrichous flagella, designated as P96 was isolated from the surface of maize roots. Strain P96 grew optimally at 28 °C, pH 7.0.

Influence of learning curve and surgeon experience on postoperative pain after laparoscopic treatment of inguinal hernias.

Introduction: Laparoscopic approach for inguinal hernia offers considerable advantages. Several risk factors for postoperative pain have been investigated, but few articles can be found about learning curve and surgeon's influence on postoperative pain after inguinal herniorrhaphy.

Patients And Methods: A prospective study was conducted including 105 patients.

Characterization of the skin microbiota in bullous pemphigoid patients and controls reveals novel microbial indicators of disease.

Introduction: Bullous pemphigoid (BP) is the most common autoimmune blistering disease. It predominately afflicts the elderly and is significantly associated with increased mortality. The observation of age-dependent changes in the skin microbiota as well as its involvement in other inflammatory skin disorders suggests that skin microbiota may play a role in the emergence of BP blistering.

[Case report of amyloid light-chain amyloidosis with periocular cutaneous involvement].

Összefoglaló. A könnyűlánc-amyloidosis ritka, multidiszciplináris jelentőségű kórkép, melynek hátterében az esetek döntő hányadában egy amyloidogen fehérje, a csontvelő kóros plazmasejtjeiben termelődő monoklonálisimmunglobulin-molekula lambda típusú könnyűláncának felszaporodása áll. A klinikai tünetek az érintett szervek függvényében igen változatosak és gyakran nem specifikusak, ezért a betegség sok esetben későn kerül felismerésre.

Influence of Mesh Fixation on the Development of Postoperative Pain after Laparoscopic Inguinal Hernia Repair: A Single Surgeon Experience.

Primary aim of the present article was to determine the relationship between mesh fixation methods and the occurrence of postoperative pain after laparoscopic inguinal hernia repair. 101 patients diagnosed with inguinal hernia benefited from elective laparoscopic treatment of the abdominal wall defect. Follow up was realized at one and three months after surgical intervention.

[True insulin allergy? Lessons from a case of suspected insulin allergy].

Today, insulin hypersensitivity reactions are rare side effects of insulin therapy. In two-thirds of the suspected insulin allergy cases, the clinical symptoms are not related to insulin. The authors report the case of a 64-year-old female patient, by whom lymphocyte tarnsformation test (LTT) has been used to elucidate the background of allergic symptoms developed during insulin therapy.

Smoothened receptor inhibitor vismodegib for the treatment of basal cell carcinoma: a retrospective analysis of efficacy and side effects.

Smoothened receptor inhibitor vismodegib is indicated to treat locally advanced basal cell carcinoma (laBCC) and lesions in nevoid basal cell carcinoma syndrome (NBCCS). We treated 11 patients - including four NBCCS and seven laBCC patients - with vismodegib at our department. Complete remission was achieved in three cases, without relapse after discontinuation.

Biological evaluation of microbial toxin degradation by microinjected zebrafish (Danio rerio) embryos.

The use of microinjection of newly fertilized zebrafish eggs as an appropriate tool for qualifying the biodetoxification properties of toxin-degrading microbes was investigated. Ochratoxin A (OTA), bacterial degradation products of OTA and bacterial metabolites of the Cupriavidus basilensis ŐR16 strain were microinjected. Results showed that variations in the injected droplet size, and thus treatment concentrations, stayed within ±20%, moreover embryo mortality did not exceed 10% in controls, that is in accordance with the recommendations of the OECD 236 guideline.

Formation of Metal-Cyanide Complexes in Deliquescent Airborne Particles: A New Possible Sink for HCN in Urban Environments.

Hydrogen cyanide is a ubiquitous gas in the atmosphere and a biomass burning tracer. Reactive gases can be adsorbed onto aerosol particles where they can promote heterogeneous chemistry. In the present study, we report for the first time on the measurement and speciation of cyanides in atmospheric aerosol.

Sirt6 alters adult hippocampal neurogenesis.

Sirtuins are pleiotropic NAD+ dependent histone deacetylases involved in metabolism, DNA damage repair, inflammation and stress resistance. SIRT6, a member of the sirtuin family, regulates the process of normal aging and increases the lifespan of male mice over-expressing Sirt6 by 15%. Neurogenesis, the formation of new neurons within the hippocampus of adult mammals, involves several complex stages including stem cell proliferation, differentiation, migration and network integration.

Predictive factors for postoperative deformity in thoracolumbar burst fractures: a statistical approach.

Objective: To assess, using statistical analysis, if and to what extent the final outcome of surgical treatment for burst fractures depends on operation type, fracture level and initial deformity severity.

Methods: A database of 287 patients with single-vertebral-level thoracic and lumbar spine fractures analysed using simple and multiple linear regression analyses models. The dependent variable was last follow-up (LFU) kyphotic angle and the predictor variables were operation type [anterior approach (AA), posterior short-segment fixation (PSSF) and posterior monosegmental fixation (PMF)], fracture level (T11-L1, L2-L3 and L4-L5) and preoperative kyphotic angle.

Reduction in absorption of gallium maltolate in adult horses following oral administration with food: chemistry and pharmacokinetics.

Gallium (Ga) is under study for the treatment of osteolytic disorders in equines. Previous studies indicate that oral gallium maltolate (GaM) would provide a higher bioavailability than oral Ga salts. However, oral administration to adult horses of 2 mg/kg of GaM, in the form of a solution mixed with food, did not lead to detectable Ga levels in plasma.

Ultratrace determination of total and available cyanides in industrial wastewaters through a rapid headspace-based sample preparation and gas chromatography with nitrogen phosphorous detection analysis.

A new analytical method for the determination of both available (free and weak acid dissociable, WAD) and total cyanides in industrial wastewaters has been developed. It is based on the static headspace (HS) sampling procedure followed by a GC separation and the selective nitrogen-phosphorous detection (NPD), in which different thermal treatment allows the speciation of total and available cyanides. Detection limits (0.

Vascular malformation of the glans penis successfully treated with Nd:YAG laser.

Penile venous malformations are uncommon and those located on the glans are even rarer. Treatment of venous malformations of the glans is currently controversial. Neodynium (Nd):YAG laser treatment may be a useful option.

UHPLC-DAD method for the determination of neonicotinoid insecticides in single bees and its relevance in honeybee colony loss investigations.

In the understanding of colony loss phenomena, a worldwide crisis of honeybee colonies which has serious consequences for both apiculture and bee-pollination-dependent farm production, analytical chemistry can play an important role. For instance, rapid and accurate analytical procedures are currently required to better assess the effects of neonicotinoid insecticides on honeybee health. Since their introduction in agriculture, neonicotinoid insecticides have been blamed for being highly toxic to honeybees, possibly at the nanogram per bee level or lower.

Connective tissue nevi in children: institutional experience and review.

Background: Connective tissue nevi (CTN) are circumscribed hamartomas of the skin in which there is an abnormal mixture of normal components of the dermis that may be sporadic or associated with syndromes such as Buschke-Ollendorff, tuberous sclerosis, and Proteus.

Objective: We sought to specify the clinical and histologic features of CTN in childhood and to propose a diagnostic approach and updated classification.

Methods: This was a retrospective study in a tertiary pediatric outpatient population, accessing clinical and histopathological records.

Assessment of the environmental exposure of honeybees to particulate matter containing neonicotinoid insecticides coming from corn coated seeds.

Since seed coating with neonicotinoid insecticides was introduced in the late 1990s, European beekeepers have reported severe colony losses in the period of corn sowing (spring). As a consequence, seed-coating neonicotinoid insecticides that are used worldwide on corn crops have been blamed for honeybee decline. In view of the currently increasing crop production, and also of corn as a renewable energy source, the correct use of these insecticides within sustainable agriculture is a cause of concern.

Long-term stability of self-assembled monolayers on electropolished L605 cobalt chromium alloy for stent applications.

Commercially available drug-eluting stents have the potential to induce inflammatory and hypersensitive adverse reactions due to their polymer coating. The use of self assembled monolayers (SAMs) as an alternate drug delivery platform for stents has recently been demonstrated. In this study, the formation and stability of phosphonic acid SAMs were investigated using the material and surface preparation commonly used to make ultra-thin stent struts-electropolished L605 Cobalt Chromium (CoCr) alloy.

Rapid analysis of neonicotinoid insecticides in guttation drops of corn seedlings obtained from coated seeds.

Regarding the hypothesis that neonicotinoid insecticides used for seed coating of agricultural crops - mainly corn, sunflower and seed rape - are related to the extensive death of honey bees, the phenomenon of corn seedling guttation has been recently considered as a possible route of exposure of bees to these systemic insecticides. In the present study, guttation drops of corn plants obtained from commercial seeds coated with thiamethoxam, clothianidin, imidacloprid and fipronil have been analyzed by an optimized fast UHPLC-DAD procedure showing excellent detection limits and accuracy, both adequate for the purpose. The young plants grown both in pots - in greenhouse - and in open field from coated seeds, produced guttation solutions containing high levels of the neonicotinoid insecticides (up to 346 mg L(-1) for imidacloprid, 102 mg L(-1) for clothianidin and 146 mg L(-1) for thiamethoxam).

Delivery of paclitaxel from cobalt-chromium alloy surfaces without polymeric carriers.

Polymer-based carriers are commonly used to deliver drugs from stents. However, adverse responses to polymer coatings have raised serious concerns. This research is focused on delivering drugs from stents without using polymers or any carriers.

Long-term stability of self-assembled monolayers on 316L stainless steel.

316L stainless steel (316L SS) has been extensively used for making orthopedic, dental and cardiovascular implants. The use of phosphonic acid self-assembled monolayers (SAMs) on 316L SS has been previously explored for potential biomedical applications. In this study, we have investigated the long-term stability of methyl (-CH(3)) and carboxylic acid (-COOH)-terminated phosphonic acid SAMs on 316L under physiological conditions.

Human aortic endothelial cell response to 316L stainless steel material microstructure.

The role of metal microstructure (e.g. grain sizes) in modulating cell adherence behavior is not well understood.

Radiographs interpretation by forensic pathologists: a word of warning.

In child deaths investigation, radiologic examination is particularly important in the diagnosis of child abuse. In the province of Quebec, Canada, all autopsies for suspicious deaths are performed at a centralized forensic laboratory where, because of budget restrictions, forensic pathologists rely on their own knowledge for radiographs interpretations. To assess the validity of this radiologic examination by nonradiologist forensic specialist, we reviewed all cases of child death on a 1-year period.

Drug delivery from gold and titanium surfaces using self-assembled monolayers.

Currently available drug-eluting stents (DES) use polymers for coating and releasing drugs. Increasing evidence suggests that inflammatory and hypersensitive reactions are caused by such polymer coatings. This study focused on developing new techniques for delivering drugs directly from metal implant surfaces.

Stability of self-assembled monolayers on titanium and gold.

Methyl- and hydroxyl-terminated phosphonic acid self-assembled monolayers (SAMs) were coated on Ti from aqueous solution. Dodecyl phosphate and dodecyltrichlorosilane SAMs were also coated on Ti using solution-phase deposition. The stability of SAMs on Ti was investigated in Tris-buffered saline (TBS) at 37 degrees C using X-ray photoelectron spectroscopy, contact angle goniometry, and atomic force microscopy.