Genetic diversity and distinction of and in traditional Montenegrin brine cheeses and salamis.

Introduction: is a widespread acid-lactic bacterium found in the environment, humans, and animal microbiota, and it also plays a role in the production of traditional food. However, the worldwide emergence of multidrug-resistant strains represents a major public health threat and is the primary reason that the genus is not recommended for the Qualified Presumption of Safety (QPS) list of the European Food Safety Authority (EFSA), raising concerns about its presence in food products.

Methods: In this study, 39 and 5 isolates were obtained from artisanal brine cheeses and dry sausages, sourced from 21 different Montenegrin producers.

Climbing the longevity pyramid: overview of evidence-driven healthcare prevention strategies for human longevity.

Longevity medicine is an emerging and iterative healthcare discipline focusing on early detection, preventive measures, and personalized approaches that aim to extend healthy lifespan and promote healthy aging. This comprehensive review introduces the innovative concept of the "." This conceptual framework delineates progressive intervention levels, providing a structured approach to understanding the diverse strategies available in longevity medicine.

A comprehensive study on the autochthonous microbiota, volatilome, physico-chemical, and morpho-textural features of Montenegrin Njeguški cheese.

The present study aims to deepen the knowledge of the microbiota, gross composition, physico-chemical and morpho-textural features, biogenic amines content and volatilome of Njeguški cheese, one of the most popular indigenous cheeses produced in Montenegro. Cheese samples were collected in duplicate from three different batches produced by three Montenegrin artisan producers. For the first time, the microbiota of Njeguški cheese was investigated using both culture-dependent techniques and metagenomic analysis.

Surveillance and genetic characterization of in the food chain in Montenegro during the period 2014-2022.

Introduction: is an ubiquitous foodborne pathogen that represents a serious threat to public health and the food industry.

Methods: In this study Whole Genome Sequencing (WGS) was used to characterize 160 isolates obtained from 22,593 different food sources in Montenegro during the years 2014-2022.

Results: Isolates belonged to 21 different clonal complexes (CCs), 22 sequence types (STs) and 73 core genome multilocus sequence types (cgMLST) revealing a high diversity.

Investigating the Relationship between Body Mass Index, Cholesterol, and Cancer in United States Adults: A Cross-Sectional Study.

The purpose of this cross-sectional study was to investigate the relationship between Body Mass Index (BMI), cholesterol, and cancer in United States (US) adults. Data were collected from the 2020 Medical Expenditure Panel Survey (MEPS). Eligible participants were US adults (≥18 years) with data on BMI, cholesterol, and cancer status, who were alive at the end of the data collection period.

Population genomics and morphological data bridge the centuries of cyanobacterial taxonomy along the continuum of species.

The filamentous cyanobacterium is among the most important global primary producers, especially in hot and cold desert ecosystems. This taxon represents a continuum consisting of a minimum of 12 distinct species with varying levels of gene flow and divergence. The notion of a species continuum is poorly understood in most lineages but is especially challenging in cyanobacteria.

Complete genome of CoE-159-22, isolated from traditionally produced Montenegrin cheese.

() was recently described as a new species isolated from copper globemallow (). Here, we report the complete genome of CoE-159-22, which was obtained from traditionally produced Montenegrin cheese.

Cadmium and lead in fresh vegetables and vegetable products sold on the local market in the Republic of Serbia, 2018-2021.

Cadmium (Cd) and lead (Pb) concentrations were determined in samples of 31 species of vegetables ( = 719) and in vegetable products ( = 17), collected during the period January 2018 to September 2021. These originated from 33 countries, including Serbia. The samples were analysed by inductively coupled plasma - optical emission spectrometry (ICP-OES).

sp. nov., isolated from artisanal Montenegrin dry sausage.

A novel, Gram-positive, facultative anaerobe, coccoid and non-motile bacterium, designated as CoE-012-22 was isolated from dried beef sausage (the original name in Montenegro is Govedji Kulen) manufactured in the municipality of Rozaje (Montenegro) in 2021. Cells of this strain were oxidase- and catalase-negative. Growth occurred at 4-50 °C, at pH 5.

Cadmium and lead in fresh fruits and fruit products sold on the local market in the Republic of Serbia, 2018-2021.

Cadmium (Cd) and lead (Pb) concentrations were determined in 737 samples of 39 species of fruits and of processed fruits ( = 13), collected during the period January 2018 to September 2021. Fruits and fruit products originated from 47 countries, including Serbia. Samples were analysed by inductively coupled plasma - optical emission spectrometry (ICP-OES).

Cadmium, lead, mercury and arsenic in fresh vegetables and vegetable products intended for human consumption in the Republic of Serbia, 2015-2017.

The concentrations of cadmium (Cd), lead (Pb), mercury (Hg) and arsenic (As) were determined in 455 samples of 27 species of vegetables and 28 different processed vegetables collected during the period from January 2015 to December 2017. Vegetables (n = 387) and vegetable products (n = 68) originated from 31 countries, including Serbia. The samples were analysed by inductively coupled plasma - optical emission spectrometry (ICP-OES).

The Ability of Streptococcus thermophilus BT01 to Modulate Urease Activity in Healthy Subjects' Fecal Samples Depends on the Biomass Production Process.

Scope: This study evaluates how manufacturing conditions of probiotic biomass production, using two different cryoprotectants, Cryo-A and Cryo-B, can affect Streptococcus thermophilus BT01 in vivo gastrointestinal tract survival and its ability to modulate the level of urease activity in fecal samples of healthy subjects.

Methods And Results: A randomized controlled cross-over study is carried out on 20 adult healthy subjects to evaluate total and viable loads, persistence of S. thermophilus BT01, and urease activity in fecal samples.

Draft Genome Sequence of Enterococcus dispar CoE-457-22, Isolated from Traditionally Produced Montenegrin Dry Sausage.

Enterococcus dispar was isolated for the first time from synovial fluid and stool cultures and described as a new species in 1991. Here, we report the genome of E. dispar CoE-457-22, which was obtained from traditionally produced Montenegrin dry sausage (sudzuk).

Cadmium, lead, mercury, and arsenic in fresh fruits and fruit products intended for human consumption in the Republic of Serbia, 2015-2017.

The European Commission has established legislation for toxic metals in certain foodstuffs in order to protect public health already in 1993. After several amendments, new maximum levels for cadmium and lead in certain foods were set as per 30 and 31 August 2021 for lead and cadmium, respectively. The new Serbian Regulation on "maximum levels of certain contaminants in food" is fully harmonised with this European legislation.

Footprint of the plastisphere on freshwater zooplankton.

Changes in the functional groups of zooplankton were studied in autumn in a temperate floodplain lake (Lake Sakadaš, Kopački Rit Nature Park, Croatia) and in the Drava River (in the Croatian part of the river). Various abiotic parameters as well as available food sources (phytoplankton and microphytes (algae and cyanobacteria) developing on epixylon, epilithon and artificially introduced microplastics called "plastisphere") were also studied. The lake was hydrologically isolated from the main river during the study, while the water level of the Drava River fluctuated, resulting in larger variations in limnological parameters.

Plastisphere development in relation to the surrounding biotic communities.

To study the early colonization processes, polyethylene terephthalate (PET) microfragments were immersed in Lake Sakadaš and the Drava River and sampled weekly together with the surrounding biotic communities - phytoplankton, zooplankton, epixylon in the lake and epilithon in the river. At the end of the study, a rise in water level occurred in the river, which altered the environmental conditions and plankton communities. In studied environments, all of the sampled biotic communities were diverse and abundant.

In Streptococcus thermophilus, Ammonia from Urea Hydrolysis Paradoxically Boosts Acidification and Reveals a New Regulatory Mechanism of Glycolysis.

Streptococcus thermophilus is widely used in the dairy industry for the manufacturing of fermented milk and cheeses and probiotic formulations. S. thermophilus evolved from closely phylogenetically related pathogenic streptococci through loss-of-function events counterbalanced by the acquisition of relevant traits, such as lactose and urea utilization for the adaptation to the milk environment.

Genetic Diversity of Isolates from Traditional Montenegrin Brine Cheese.

In many dairy products, spp. is a natural part of non-starter lactic acid bacteria (NSLAB) accounting for flavor development. However, data on the genomic diversity of spp.

Genome Sequences of Lactococcus garvieae and Lactococcus petauri Strains Isolated from Traditional Montenegrin Brine Cheeses.

Lactococcus garvieae and Lactococcus petauri cause lactococcosis in fish. Both species have also been isolated from various food products and are considered emerging zoonotic pathogens. Here, we report the genomes of L.

Effect of Tympanostomy Tube Placement on Intraoperative Auditory Brainstem Response.

Background: Intraoperative auditory brainstem response (ioABR) testing under general anesthesia is commonly performed on children when sleep-deprived ABR and behavioral testing are not reliable or feasible. Several studies have reported potential confounding results when tube insertion is combined with ABR testing.

Purpose: This article evaluates whether a temporary threshold shift (TTS) occurs following placement of tympanostomy tubes (TTs) in children who undergo ioABR testing.

Role of cochlear synaptopathy in cytomegalovirus infected mice and in children.

Objectives: Determine whether a murine model of cytomegalovirus (CMV) and CMV- infected children show evidence of synaptopathy.

Study Design: Murine model of CMV infection and case series.

Subjects And Methods: C57 BL/6 mice were inoculated with murine-CMV (mCMV).

New Formulation towards Healthier Meat Products: L. Essential Oil as Alternative for Sodium Nitrite in Dry Fermented Sausages.

The effect of L. essential oil (JEO) addition at concentrations of 0.01, 0.

: To Survive, or Not to Survive the Gastrointestinal Tract, That Is the Question!

The probiotic market is increasing world-wide as well as the number of products marketed as probiotics. Among the latter, many products contain strains at several dosages. However, the scientific evidence that should support the probiotic status of those strains is often contradictory.

Draft Genome Sequences of Five Enterococcus faecium Isolates from Traditional Montenegrin Brine Cheese.

is a multifaceted bacterial species. It is part of the natural human microbiota, it grows in a variety of traditional foods, and emerging multiresistant clones are a leading cause of nosocomial infections. Here, we present draft genomes of five isolates originating from traditional Montenegrin brine cheeses.