Publications by authors named "Martine Pittet"
Biosynthesis and function of GPI proteins in the yeast Saccharomyces cerevisiae.
Biochim Biophys Acta
March 2007
Article Synopsis
- - Saccharomyces cerevisiae, like other eukaryotes, uses a GPI anchoring system to attach proteins to membranes, with many of these proteins subsequently integrated into the cell wall via covalent bonds to glucans.
- - The GPI biosynthetic pathway is essential for yeast growth and survival, involving 12 steps and 23 genes, of which 19 are critical for the process.
- - With around 60 predicted GPI proteins, some function in biosynthesis and cell wall maintenance, while others provide structure or support cell adhesion, making S. cerevisiae an excellent model for studying GPI processes and fungal cell wall formation.
CWH8/YGR036c of Saccharomyces cerevisiae has been identified as a dolichylpyrophosphate (Dol-PP) phosphatase that removes a phosphate from the Dol-PP generated by the oligosaccharyltransferase (OST), while it adds N-glycans to nascent glycoproteins in the endoplasmic reticulum (ER). Lack of CWH8 was proposed to interrupt the so called dolichol (Dol) cycle by trapping Dol in the form of Dol-PP in the ER lumen. Indeed, cwh8Delta mutants display a severe deficiency in N-glycosylation.
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- The study looked at how interleukin-4 (IL-4) affects cancer cells and how it interacts with insulin-like growth factor-1 (IGF-1).
- Both IL-4 and IGF-1 use similar pathways in cells to signal and promote changes, but IL-4 only partially activates a key enzyme while IGF-1 fully activates it.
- IL-4 can slow down cell growth significantly, working even when IGF-1 is present, and it seems to do this by changing gene expression rather than making cells resistant to insulin or growth factors.
Rapid effects of glucose on the insulin signaling of endothelial NO generation and epithelial Na transport.
Am J Physiol Endocrinol Metab
January 2002
Insulin resistance is associated with deficits in glucose metabolism. We tested whether the vascular and renal responses to insulin might contribute to insulin resistance. Generation of endothelial-derived vasodilator nitric oxide (NO), estimated after a 2-h period of insulin stimulation, was inhibited in the presence of high glucose.
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