A Structural Analysis of the Angucycline-Like Antibiotic Auricin from Subsp. CCM 3239 Revealed Its High Similarity to Griseusins.

We previously identified the gene cluster in subsp. CCM 3239 (formerly CCM 3239), which is responsible for the production of the angucycline-like antibiotic auricin (). Preliminary characterization of revealed that it possesses an aminodeoxyhexose d-forosamine and is active against Gram-positive bacteria.

Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells.

We report results showing that the silencing of carbonic anhydrase I (siCA1) in prostatic (PC3) tumour cells has a significant impact on exosome formation. An increased diameter, concentration and diversity of the produced exosomes were noticed as a consequence of this knock-down. The protein composition of the exosomes' cargo was also altered.

Salidroside, a Chemopreventive Glycoside, Diminishes Cytotoxic Effect of Cisplatin in Vitro.

Natural products represent the source or the inspiration for the majority of the active ingredients of medicines because of their structural diversity and a wide range of biological effects. Our aims in this study were (i) to synthesize enzymatically salidroside (SAL), the most effective phenylethanoid glycoside in Rhodiola species; (ii) to examine its antioxidant capacity using cell-free assays (reducing power, DPPH radicals scavenging and Fe -chelating assays); (iii) to assess its DNA-protective potential on plasmid DNA (DNA topology assay) and in HepG2 cells (comet assay) damaged by Fe ions and hydrogen peroxide, respectively; and (iv) to investigate the effects of SAL, cisplatin (CDDP) and combined treatments of SAL + CDDP on cell viability (MTT test), level of DNA damage (comet assay), proliferation, cell cycle (flow cytometry) and the expression of signalling molecules associated with cell growth and apoptotic pathways (Western immunoblotting). We found out that SAL manifested low antioxidant and DNA-protective capacity in all assays used.

Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells.

Hyperforin (HF), a substance that accumulates in the leaves and flowers of L. (St. John's wort), consists of a phloroglucinol skeleton with lipophilic isoprene chains.