Publications by authors named "Martina Zanova"
Article Synopsis
- Dicer endonucleases produce small RNAs for microRNA (miRNA) and RNA interference (RNAi) pathways, playing a crucial role in gene regulation.
- The DExD/H helicase domain of Dicer is vital for high-fidelity miRNA biogenesis, with its complete absence being lethal and its ATPase function being dispensable.
- Structural studies show that the DExD/H domain helps lock Dicer in a closed state for miRNA precursor selection, while mutations can lead to reduced selectivity and activation of the RNAi pathway.
Transcription elongation factor Spt6 associates with RNA polymerase II (Pol II) and acts as a histone chaperone, which promotes the reassembly of nucleosomes following the passage of Pol II. The precise mechanism of nucleosome reassembly mediated by Spt6 remains unclear. In this study, we used a hybrid approach combining cryo-electron microscopy and small-angle X-ray scattering to visualize the architecture of Spt6 from Saccharomyces cerevisiae.
View Article and Find Full Text PDF