Publications by authors named "Martina Winter"
3D printing has become widespread for the manufacture of parts in various industries and enabled radically new designs. This trend has not spread to bioprocess development yet, due to a lack of material suitable for the current workflow, including sterilization by autoclaving. This work demonstrates that commercially available heat temperature stable poly-lactic acid (PLA) can be used to easily manufacture novel bioreactor vessels with included features like harvest tubes and 3D printed spargers.
View Article and Find Full Text PDFThe aim of this study was the development of a scalable production process for high titer (10 pfu/mL and above) recombinant baculovirus stocks with low cell line-derived impurities for the production of virus-like particles (VLP). To achieve this, we developed a high cell density (HCD) culture for low footprint cell proliferation, compared different infection strategies at multiplicity of infection (MOI) 0.05 and 0.
View Article and Find Full Text PDFArticle Synopsis
- - A new continuous protein recovery and purification system uses a moving bed concept with a special woven fabric that acts as an adsorbent material, designed based on belt conveyor principles.
- - The woven fabric demonstrated a high static protein binding capacity of 107.3 mg/g and showed good performance in a packed bed format with a dynamic binding capacity of 54.5 mg/g, even at high flow rates.
- - A prototype system successfully recovered a model protein with a productivity of 0.5 mg/cm/h and isolated a monoclonal antibody with high purity from cell culture, demonstrating efficient and selective purification in just one step.