IDENTIFICATION OF THE FGL-AMIDE ALLATOSTATIN GENE OF THE PRIMITIVE TERMITE Mastotermes darwiniensis AND THE WOODROACH Cryptocercus darwini.

Allatostatins with the C-terminal ending Tyr/Phe-Xaa-Phe-Gly-Leu/Ile-amide (FGLa/ASTs) are widespread neuropeptides with multiple functions. The gene encoding the FGLa/AST polypeptide precursor was first isolated from cockroaches and since then could be identified in many insects and crustaceans. With its strictly conserved regions in combination with variable regions the gene seems to be a good candidate for phylogenetic analyses between closely and distantly related species.

Neuropeptides affecting the transfer of juvenile hormones from males to females during mating in Spodoptera frugiperda.

In the polyandric moth, Spodopterafrugiperda, juvenile hormone (JH) is transferred from the male accessory reproductive glands (AG) to the female bursa copulatrix (BC) during copulation (see Hassanien et al., 2014). Here we used the RNA interference technique to study the role of allatoregulating neuropeptides in controlling the synthesis and transfer of JH during mating.

Putative-farnesoic acid O-methyltransferase (FAMeT) in medfly reproduction.

A gene potentially involved in juvenile hormone (JH) biosynthesis was previously identified in Ceratitis capitata as the putative-farnesoic acid O-methyltransferase (FAMeT). Since JH is involved in insect reproduction, we silenced the putative-FAMeT expression by RNA interference in Ceratitis capitata to evaluate its implication in egg production. FAMeT gene expression was knocked down in females and males after eclosion and in 1- and 2-day-old females.

Effects of temperature on reproductive output, egg provisioning, juvenile hormone and vitellogenin titres in the butterfly Bicyclus anynana.

Environmentally induced phenotypic plasticity is common in nature. Hormones, affecting multiple traits and signaling to a variety of distant target tissues, provide a mechanistic link between environments, genes and trait expression, and may therefore well be involved in the regulation phenotypic plasticity. Here, we investigate whether in the tropical butterfly Bicyclus anynana temperature-mediated plasticity in egg size and number, with fewer but larger eggs produced at lower temperatures and vice versa, is under control of juvenile hormone, and whether different temperatures cause differences in egg composition.

RNA interference with the allatoregulating neuropeptide genes from the fall armyworm Spodoptera frugiperda and its effects on the JH titer in the hemolymph.

The juvenile hormone (JH) titer was measured by liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization (ESI). Three JH homologs, the JH I-III were detected in various amounts in larvae, prepupae and virgin adult females of Spodoptera frugiperda. In penultimate larvae, the JH II and III titers were relatively high, but decreased continuously during the 3 days of that stage, whereas JH I was detectable at low amounts only on the first 2 days.

RNA interference suggests sulfakinins as satiety effectors in the cricket Gryllus bimaculatus.

In the Mediterranean field cricket, Gryllus bimaculatus, the action of sulfakinin (SK) gene expression on food intake, food transport in the gut and carbohydrate digestion (alpha-amylase activity) was investigated by using the RNA interference (RNAi) method. Injection of SK double-stranded (ds) RNA into the abdomen of female adults and last instar larvae led to a systemic silencing of the SK gene, as was shown by RT-PCR studies. In adults, suppression of SK gene expression was effective from the first day after injection up to at least the third day.

Functional analysis of the allatostatin-A type gene in the cricket Gryllus bimaculatus and the armyworm Spodoptera frugiperda.

Double-stranded RNA (dsRNA) gene interference is an efficient method to silence gene expression in a sequence specific manner. Here we show, that dsRNA targeting the allatostatin (AS)-A type (FGL/I/V-amide) gene of Gryllus bimaculatus (Ensifera, Gryllidae) and Spodoptera frugiperda (Lepidoptera, Noctuidae) injected into freshly moulted larvae or adult crickets and moths produced a rapid and long-lasting reduction in the mRNA levels in various tissues. The effect lasted up to 7 days.

Characterization of a novel peptide with allatotropic activity in the fall armyworm Spodoptera frugiperda.

A cDNA that encodes 53 amino acids, including one copy of the RVRGNPISCF-OH peptide, was cloned from Spodoptera frugiperda. This peptide strongly stimulates the synthesis and release of juvenile hormone (JH) in vitro by the corpora allata (CA) of S. frugiperda and was code-named Spofr-AT 2.

Type-A allatostatins from the fall armyworm, Spodoptera frugiperda: molecular cloning, expression and tissue-specific localization.

The gene encoding the Spodoptera frugiperda allatostatin type-A peptide family (Y/FXFGL-amides) was isolated from S. frugiperda brain cDNA. The gene encodes a precursor of 231 amino acids containing nine (or ten) Y/FXFGL-a peptides that are tandemly arranged in three blocks.

Expression and localization of the Spodoptera frugiperda allatotropin (Spofr-AT) and allatostatin (Spofr-AS) genes.

Manduca sexta allatotropin and allatostatin were the first corpora allata (CA) regulating neuropeptides identified from Lepidoptera. Recently, we cloned the allatotropin (Spofr-AT) and the allatostatin (Spofr-AS) genes from the fall armyworm Spodoptera frugiperda. Using one-step RT-PCR for semi-quantification of the gene expression, we now demonstrate that three mRNA isoforms of the Spofr-AT gene and the Spofr-AS gene are expressed in brain, digestive tract, and reproductive organs of larvae, pupae, and adults in a time- and tissue-specific manner.