Publications by authors named "Martin Wolfring"
Article Synopsis
- Non-ionic surfactants like polysorbate 20, polysorbate 80, and poloxamer188 are commonly used in parenteral protein formulations, and their quantification is important due to their role in visible particle formation.
- A new mixed mode chromatography method with single quad mass detection has been developed to effectively quantify surfactants and their degradation patterns in highly concentrated protein formulations.
- This method demonstrated strong performance characteristics, including high linearity, precision, and accuracy, meeting current pharmaceutical standards as outlined in ICH Q2.
Bioorthogonal chemistry holds great potential to generate difficult-to-access protein-protein conjugate architectures. Current applications are hampered by challenging protein expression systems, slow conjugation chemistry, use of undesirable catalysts, or often do not result in quantitative product formation. Here we present a highly efficient technology for protein functionalization with commonly used bioorthogonal motifs for Diels-Alder cycloaddition with inverse electron demand (DA ).
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