BioSAXS Measurements Reveal That Two Antimicrobial Peptides Induce Similar Molecular Changes in Gram-Negative and Gram-Positive Bacteria.

Two highly active short broad-spectrum AMPs (14D and 69D) with unknown mode of action have been investigated in regards to their effect against the Gram-negative bacteria Escherichia and the Gram-positive bacteria methicillin-resistant (MRSA). Minimal inhibitory concentration (MIC) measurements using a cell density of 10 cfu/ml resulted in values between 16 and 32 µg/ml. Time-kill experiments using 10 cfu/ml revealed complete killing, except for 69D in combination with MRSA, where bacterial load was reduced a million times.