Is Protein BLAST a thing of the past?

Will protein structure search tools like AlphaFold replace protein sequence search with BLAST? We discuss the promises, using structure search for remote homology detection, and why protein BLAST, as the leading sequence search tool, should strive to incorporate structural information

The influence of storage temperature during machine perfusion on preservation quality of marginal donor livers.

Background: Although non-heart-beating donors have the potential to increase the number of available organs, the livers are used very seldom because of the risk of primary non-function. There is evidence that machine perfusion is able to improve the preservation of marginal organs, and therefore we evaluated in our study the influence of the perfusate temperature during oxygenated machine perfusion on the graft quality.

Methods: Livers from male Wistar rats were harvested after 60-min warm ischemia induced by cardiac arrest.