Exercise training-induced speeding of kinetics is not intensity domain-specific or correlated with indices of exercise performance.

Purpose: This study examined the effect of 3 and 6 weeks of intensity domain-based exercise training on kinetics changes and their relationship with indices of performance.

Methods: Eighty-four young healthy participants (42 M, 42 F) were randomly assigned to six groups (14 participants each, age and sex-matched) consisting of: continuous cycling in the (1) moderate (MOD)-, (2) lower heavy (HVY1)-, and (3) upper heavy-intensity (HVY2)- domain; interval cycling in the (4) severe-intensity domain (i.e.

Identification of a Resistance Exercise-Specific Signaling Pathway that Drives Skeletal Muscle Growth.

A human model of unilateral endurance versus resistance exercise, in conjunction with deep phosphoproteomic analyses, was used to identify exercise mode-specific phosphorylation events. Among the outcomes, a resistance exercise-specific cluster of events was identified, and a multitude of bioinformatic- and literature-based predictions suggested that this was mediated by prolonged activation of a pathway involving MKK3b/6, p38, MK2, and mTORC1. Follow-up studies in humans and mice provide consistent support for the predictions and also revealed that resistance exercise-induced signaling through MKK3b and the induction of protein synthesis are highly correlated events (R = 0.

Sex-based differences in hematological values after normalization to body mass or fat-free mass in adults matched for aerobic fitness.

Blood properties influence aerobic exercise performance. While vascular volumes and hemoglobin mass (Hb) are elevated in trained individuals, evidence of sex differences in vascular volumes is equivocal due to inadequate matching of aerobic fitness between males and females. This cross-sectional study aimed to compare hematological values normalized to body mass (BM) and fat-free mass (FFM) between males ( = 45) and females ( = 34) matched for aerobic fitness (V̇Omax) normalized to FFM (mL∙kg FFM∙min ).

The Effect of Duration on Performance and Perceived Fatigability During Acute High-Intensity Interval Exercise in Young, Healthy Males and Females.

Few studies have explored the kinetics of performance and perceived fatigability during high-intensity interval training, despite its popularity. We aimed to characterize the kinetics of fatigability and recovery during an 8 × 4-min HIIT protocol, hypothesizing that most muscle function impairment would occur during the initial four intervals. Fifteen healthy males and females (mean ± standard deviation; age = 26 ± 5 years, V̇Omax = 46.

Counterweight mass influences single-leg cycling biomechanics.

Introduction: Single-leg cycling is a commonly used intervention in exercise physiology that has applications in exercise training and rehabilitation. The addition of a counterweight to the contralateral pedal helps single-leg cycling mimic cycling patterns of double-leg cycling. To date, no research has tested (a) the influence of a wide range of counterweight masses on a person's cycling biomechanics and (b) the optimal counterweight mass to emulate double-leg cycling.

Effects of acute simulated altitude on the maximal lactate steady state in humans.

We sought to determine the effects of acute simulated altitude on the maximal lactate steady state (MLSS) and physiological responses to cycling at and 10 W above the MLSS-associated power output (PO) (MLSS and MLSS, respectively). Eleven (4 females) participants (means [SD]; 28 [4] yr; V̇o: 54.3 [6.

The influence of sex, hemoglobin mass, and skeletal muscle characteristics on cycling critical power.

Critical power (CP) represents an important threshold for exercise performance and fatiguability. We sought to determine the extent to which sex, hemoglobin mass (Hb), and skeletal muscle characteristics influence CP. Before CP determination (i.

Identifying physiological determinants of 800 m running performance using post-exercise blood lactate kinetics.

Purpose: The aims of the present study were to investigate blood lactate kinetics following high intensity exercise and identify the physiological determinants of 800 m running performance.

Methods: Fourteen competitive 800 m runners performed two running tests. First, participants performed a multistage graded exercise test to determine physiological indicators related to endurance performance.

Acute performance fatigability following continuous versus intermittent cycling protocols is not proportional to total work done.

Classical training theory postulates that performance fatigability following a training session should be proportional to the total work done (TWD); however, this notion has been questioned. This study investigated indices of performance and perceived fatigability after primary sessions of high-intensity interval training (HIIT) and constant work rate (CWR) cycling, each followed by a cycling time-to-task failure (TTF) bout. On separate days, 16 participants completed an incremental cycling test, and, in a randomized order, (i) a TTF trial at 80% of peak power output (PPO), (ii) an HIIT session, and (iii) a CWR session, both of which were immediately followed by a TTF trial at 80% PPO.

Performance and perceived fatigability across the intensity spectrum: role of muscle mass during cycling.

The role of muscle mass in modulating performance and perceived fatigability across the entire intensity spectrum during cycling remains unexplored. We hypothesized that at task failure (T), muscle contractile function would decline more following single- (SL) versus double-leg (DL) cycling within severe and extreme intensities, but not moderate and heavy intensities. After DL and SL ramp-incremental tests, on separate days, 11 recreationally active males (V̇o: 49.

Dose-Responsive Effects of Iron Supplementation on the Gut Microbiota in Middle-Aged Women.

Oral iron supplementation is the first-line treatment for addressing iron deficiency, a concern particularly relevant to women who are susceptible to sub-optimal iron levels. Nevertheless, the impact of iron supplementation on the gut microbiota of middle-aged women remains unclear. To investigate the association between iron supplementation and the gut microbiota, healthy females aged 40-65 years ( = 56, BMI = 23 ± 2.

Heavy-, Severe-, and Extreme-, but Not Moderate-Intensity Exercise Increase V̇o 2max and Thresholds after 6 wk of Training.

Introduction: This study assessed the effect of individualized, domain-based exercise intensity prescription on changes in maximal oxygen uptake (V̇O 2max ) and submaximal thresholds.

Methods: Eighty-four young healthy participants (42 females, 42 males) were randomly assigned to six age, sex, and V̇O 2max -matched groups (14 participants each). Groups performed continuous cycling in the 1) moderate (MOD), 2) lower heavy (HVY1), and 3) upper heavy-intensity (HVY2) domain; interval cycling in the form of 4) high-intensity interval training (HIIT) in the severe-intensity domain, or 5) sprint-interval training (SIT) in the extreme-intensity domain; or no exercise for 6) control (CON).

Effects of carbohydrate availability on cycling endurance at the maximal lactate steady state.

The impacts of carbohydrate (CHO) availability on time to task failure (TTF) and physiological responses to exercise at the maximal lactate steady state (MLSS) have not been studied. Ten participants (3 females, 7 males) completed this double-blinded, placebo-controlled study that involved a ramp incremental test, MLSS determination, and four TTF trials at MLSS, all performed on a cycle ergometer. With the use of a combination of nutritional (CHO, 7 g/kg, and placebo, PLA, 0 g/kg drinks) and exercise interventions [no exercise (REST) and glycogen-reducing exercise (EX)], the four conditions were expected to differ in preexercise CHO availability (REST > REST > EX > EX).

Use of subject-specific models to detect fatigue-related changes in running biomechanics: a random forest approach.

Running biomechanics are affected by fatiguing or prolonged runs. However, no evidence to date has conclusively linked this effect to running-related injury (RRI) development or performance implications. Previous investigations using subject-specific models in running have demonstrated higher accuracy than group-based models, however, this has been infrequently applied to fatigue.

Contraction intensity affects NIRS-derived skeletal muscle oxidative capacity but not its relationships to mitochondrial protein content or aerobic fitness.

To further refine the near-infrared spectroscopy (NIRS)-derived measure of skeletal muscle oxidative capacity in humans, we sought to determine whether the exercise stimulus intensity affected the τ value and/or influenced the magnitude of correlations with in vitro measures of mitochondrial content and in vivo indices of exercise performance. Males ( = 12) and females ( = 12), matched for maximal aerobic fitness per fat-free mass, completed NIRS-derived skeletal muscle oxidative capacity tests for the vastus lateralis following repeated contractions at 40% (τ) and 100% (τ) of maximum voluntary contraction, underwent a skeletal muscle biopsy of the same muscle, and performed multiple intermittent isometric knee extension tests to task failure to establish critical torque (CT). The value of τ (34.

Is Running Power a Useful Metric? Quantifying Training Intensity and Aerobic Fitness Using Stryd Running Power Near the Maximal Lactate Steady State.

We sought to determine the utility of Stryd, a commercially available inertial measurement unit, to quantify running intensity and aerobic fitness. Fifteen (eight male, seven female) runners (age = 30.2 [4.

The influence of skeletal muscle mitochondria and sex on critical torque and performance fatiguability in humans.

Critical torque (CT) represents the highest oxidative steady state for intermittent knee extensor exercise, but the extent to which it is influenced by skeletal muscle mitochondria and sex is unclear. Vastus lateralis muscle biopsy samples were collected from 12 females and 12 males -matched for relative maximal oxygen uptake normalized to fat-free mass (FFM) (F: 57.3 (7.

A Comparison of Critical Speed and Critical Power in Runners Using Stryd Running Power.

Purpose: Although running traditionally relies on critical speed (CS) as an indicator of critical intensity, portable inertial measurement units offer a potential solution for estimating running mechanical power to assess critical power (CP) in runners. The purpose of this study was to determine whether CS and CP differ when assessed using the Stryd device, a portable inertial measurement unit, and if 2 running bouts are sufficient to determine CS and CP.

Methods: On an outdoor running track, 10 trained runners (V˙O2max, 59.

Shorter High-Intensity Cycling Intervals Reduce Performance and Perceived Fatigability at Work-Matched but Not Task Failure.

Introduction: The intensity, duration, and distribution of work and recovery phases during high-intensity interval training (HIIT) modulate metabolic perturbations during exercise and subsequently influence the development of performance fatigability and exercise tolerance. This study aimed to characterize neuromuscular, perceptual, and cardiorespiratory responses to work-to-rest ratio-matched HIIT protocols differing in work and rest interval duration.

Methods: Twelve healthy individuals (six women) first completed a ramp incremental test to determine 90% of peak power output, and then in three randomized visits, they completed three cycling protocols to task failure at 90% of peak power output: (i) 3- to 3-min work-to-passive rest ratio HIIT (HIIT 3min ), (ii) 1- to 1-min work-to-passive rest ratio HIIT (HIIT 1min ), and (iii) constant load (CL).

A "Step-Ramp-Step" Protocol to Identify Running Speed and Power Associated with the Maximal Metabolic Steady State.

Purpose: A previously established Step-Ramp-Step (SRS) exercise protocol was able to accurately predict the work rate associated with the maximal metabolic steady state (MMSS) in cyclists. The purpose of this study was to determine whether a modified SRS protocol could predict the running speed and power associated with the MMSS.

Methods: Fifteen (8 male; 7 female) runners (V̇O 2max 54.

Physiological basis of brief, intense interval training to enhance maximal oxygen uptake: a mini-review.

Brief, intense interval training describes a style of exercise characterized by short bouts of strenuous effort interspersed with recovery periods. The method increases whole body maximal oxygen uptake (V̇o), but the underlying physiological basis is unclear. V̇o represents the functional limit of the integrative oxygen cascade, which refers to the physiological steps involved in oxygen transport and utilization from atmospheric air to mitochondrial metabolism.

Physiological responses to ramp-incremental cycling tests performed at three simulated altitudes: a randomized crossover trial.

Hypoxia negatively impacts aerobic exercise, but exercise testing in hypoxia has not been studied comprehensively. To determine the effects of simulated altitude on the gas exchange threshold (GET), respiratory compensation point (RCP), and maximal oxygen uptake (V̇Omax), 24 participants (mean [SD]; 26 [4] years; 171.6 [9.