Model-based selection of the degree of cross-linking of cation exchanger resins for an optimised separation of monosaccharides.

One of the main steps in designing preparative chromatographic separation units is the selection of a well-performing adsorbent. This is often based on expert knowledge or based on case studies of preselected adsorbents. Therefore, the selection is usually limited in terms of an optimised choice.

Simultaneous identification of reaction and inactivation kinetics of an enzyme-catalyzed carboligation.

Thiamine diphosphate (ThDP)-dependent enzymes catalyze a broad range of reactions with excellent enantioselectivity. Among these reactions, carboligations of aldehydes are of particular interest since the products, chiral hydroxy ketones, are valuable building blocks in the pharmaceutical industry. However, the substrates, for example, benzaldehyde, inactivate the biocatalysts, for example the ThDP-dependent benzaldehyde lyase from Pseudomonas fluorescens (PfBAL).