Publications by authors named "Martin Carson"
Oat crown rust is one of the most damaging diseases of oat. We identified a new source of resistance and developed KASP and TaqMan markers for selection in breeding programs. A new highly effective resistance to oat crown rust (Puccinia coronata f.
View Article and Find Full Text PDFOat crown rust, caused by f. sp. , is a major constraint to oat ( L.
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- 635 oat lines and 4561 SNP loci were analyzed to understand population structure, linkage disequilibrium (LD), and the relationship between genotypes and heading date.
- The analysis revealed that 25.3% of genetic variation could be explained by the first five principal components, but no clear structured population was found, although clustering indicated differences between spring oats and southern U.S. origins.
- Linkage disequilibrium was observed to decay slower in southern oat lines compared to spring oat lines, particularly highlighting an interesting case on linkage group Mrg28, and several linkage groups were consistently associated with heading date across different environments.
Article Synopsis
- * Researchers created three mapping populations by crossing a susceptible oat variety with two partially resistant varieties, and then evaluated these for disease severity across multiple years and locations.
- * Linkage analyses identified several quantitative trait loci (QTLs) linked to partial resistance, pinpointing specific chromosomes involved, and suggested candidate genes for further research on disease resistance based on comparisons with the rice genome.
A trade-off between a pathogen's ability to infect many hosts and its reproductive capacity on each host genotype is predicted to limit the evolution of an expanded host range, yet few empirical results provide evidence for the magnitude of such trade-offs. Here, we test the hypothesis for a trade-off between the number of host genotypes that a fungal pathogen can infect (host genotype range) and its reproductive capacity on susceptible plant hosts. We used strains of the oat crown rust fungus that carried widely varying numbers of virulence (avr) alleles known to determine host genotype range.
View Article and Find Full Text PDFArticle Synopsis
- The study addresses the challenges of creating a consensus map for the complex hexaploid oat genome (Avena sativa), including the size of the genome and scarcity of molecular markers.
- It introduces new methodologies for discovering SNPs and a novel anchoring strategy, successfully resulting in the first complete physically-anchored consensus map that includes 985 SNPs.
- The findings also highlight genetic similarities with other plants, providing tools for detailed genetic analysis and a useful framework for similar research in other complex genomes.
Background: Adaptation of pathogens to their hosts depends critically on factors affecting pathogen reproductive rate. While pathogen reproduction is the end result of an intricate interaction between host and pathogen, the relative contributions of host and pathogen genotype to variation in pathogen life history within the host are not well understood. Untangling these contributions allows us to identify traits with sufficient genetic variation for selection to act and to identify mechanisms of coevolution between pathogens and their hosts.
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- Genetic markers are essential in genomics, but challenges in oat genome complexity and lack of sequence data make finding and testing them difficult; this study aimed to address these issues by generating oat expressed sequence tag (EST) data and developing a method for SNP identification.
- Researchers created a bioinformatics pipeline that processed around 1 million sequence reads, resulting in the identification of 96 in silico SNPs, with 52 found to be polymorphic in a specific oat mapping population, validating their utility through high-resolution melting (HRM) analysis.
- The study concludes that the newly developed high-throughput SNP discovery pipeline and HRM genotyping method are effective for identifying and analyzing genetic diversity in oats, offering a straightforward approach to understanding their complex poly
The life history of Puccinia striiformis remains a mystery because the alternate host has never been identified. Inoculation of grasses using aeciospores from naturally infected Berberis chinensis and B. koreana resulted in infection on Poa pratensis, producing uredinia typical of stripe rust caused by P.
View Article and Find Full Text PDFWe report the development of 37 novel and polymorphic microsatellite markers for oat crown rust, Puccinia coronata f.sp. avenae.
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