A novel α(0) -thalassemia deletion in a Greek patient with HbH disease and β-thalassemia trait.

Objectives: To determine the molecular basis in a Greek child suspected of having HbH disease and β-thalassemia trait.

Methods:   Standard hematology, Hb electrophoresis, and HPLC. Multiplex ligation-dependent probe amplification (MLPA), direct sequencing, and breakpoint characterization by NimbleGen fine-tiling array analysis.