Evaluation of three proteins involved in the adhesion process as antigens for the detection of bovine intramammary infections.

. Fast and accurate diagnosis is one of the key strategies in the successful control of intramammary infections caused by . Immunoassays are one of the diagnostic tools that have been proposed for the detection of infection because they offer an advantage in terms of cost and are fast and easy to use compared to other diagnostic tests.

DNA barcodes of birds from northern Colombia.

DNA barcode datasets are a useful tool for conservation and aid in taxonomic identification, particularly in megadiverse tropical countries seeking to document and describe its biota, which is dropping at an alarming rate during recent decades. Here we report the barcodes for several low elevation bird species from northern Colombia with the goal to provide tools for species identification in this region of South America. We blood-sampled birds in a lowland tropical forest with various degrees of intervention using standard 3 × 12 m mist-nets.

Efficacy of high-intensity interval training compared with moderate-intensity continuous training on maximal aerobic potency in dogs: Trial protocol for a randomised controlled clinical study.

Background: High-intensity interval training (HIIT) is a more efficient method to improve exercise capacity than moderate-intensity continuous training (MICT) because of its greater physiological stimulus.

Objective: The aim of this protocol is to evaluate the efficacy of HIIT on maximal aerobic potency in dogs as compared to MICT.

Methods: This protocol is for a randomised, blinded controlled clinical trial, with three parallel groups for the purpose of demonstrating superiority.

Phenotypic Characterization and Whole Genome Analysis of a Strong Biofilm-Forming Strain Associated With Subclinical Bovine Mastitis in Colombia.

represent a serious threat to public health due to food safety, antibiotic resistance, and the potential zoonotic transmission of strains between dairy cattle and humans. Biofilm formation by results in chronicity of the infections which confers protection against the immune response and antibiotics. Likewise, biofilm allows the exchange of mobile genetic material among different strains through microbial interactions inside the matrix.

Using near-infrared spectroscopy to determine intramuscular fat and fatty acids of beef applying different prediction approaches.

This study aimed to predict fat and fatty acids (FA) contents in beef using near-infrared spectroscopy and prediction models based on partial least squares (PLS) and support vector machine regression in radial kernel (R-SVR). Fat and FA were assessed in 200 longissimus thoracis samples, and spectra were collected in reflectance mode from ground meat. The analyses were performed for PLS and R-SVR with and without wavelength selection based on genetic algorithms (GAs).

Near-Infrared Spectroscopy as a Beef Quality Tool to Predict Consumer Acceptance.

This study was conducted to evaluate the feasibility of using near-infrared spectroscopy (NIRS) to predict beef consumers' perceptions. Photographs of 200 raw steaks were taken, and NIRS data were collected (transmittance and reflectance). The steak photographs were used to conduct a face-to-face survey of 400 beef consumers.

Diagnosis of human and canine infection: development and evaluation of indirect enzyme-linked immunosorbent assays using recombinant proteins.

, a Gram-negative coccobacilli belonging to the genus , is a pathogenic bacterium that can produce infections in dogs and humans. Multiple studies have been carried out to develop diagnostic techniques to detect all zoonotic . Diagnosis of infection is challenging due to the lack of highly specific and sensitive diagnostic assays.

[Characterization of the genetic variability of field strains of Brucella canis isolated in Antioquia].

Brucella canis is a facultative intracellular pathogen responsible for canine brucellosis, a zoonotic disease that affects canines, causing abortions and reproductive failure; and the production of non-specific symptoms in humans. In 2005 the presence of B. canis in Antioquia was demonstrated and the strains were identified as type 2.

Comparison of Brucella canis genomes isolated from different countries shows multiple variable regions.

Brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years. In this study, we report the sequencing, annotation and analysis of the genome of Brucella canis strain Oliveri isolated from a dog in a breeding kennel in Medellín, Colombia, South America. Whole genome shotgun sequencing was carried out using the ROCHE 454 GS FLX Titanium technology at the National Center for Genomic Sequencing-CNSG in Medellin, Colombia.

[Factors associated with Brucella canis seropositivity in kennels of two regions of Antioquia, Colombia].

The objectives of this study were to determine Brucella canis seroprevalence in dogs and in humans living near kennels and to explore risk factors associated with seropositivity. Twenty kennels were included in a serological survey with RSAT-2ME, and samples were collected from 428 dogs and 91 humans. An interview was applied to determine risk factors, and the data were analyzed using logistic regression.

Identification of the virB operon genes encoding the type IV secretion system, in Colombian Brucella canis isolates.

Canine brucellosis is a zoonotic disease caused by Brucella canis. The establishment of intracellular replicative niches of B. canis is mediated by proteins secreted by the type IV secretion system, which is encoded by the virB operon.

Serological trail of Brucella infection in an urban slum population in Brazil.

Introduction: Brucellosis is a re-emerging zoonosis with new cases reported each year in many Latin American countries, but it is mostly under-recognized. This study presents a serological investigation of infection with Brucella abortus and Brucella canis in a poor urban community in the city of Salvador, Brazil.

Methodology: Human sera (n = 180) were randomly selected from 3,171 samples taken from healthy individuals during 2003-2004 and tested with C-ELISA for B.

Permanent genetic resources added to Molecular Ecology Resources Database 1 October 2010-30 November 2010.

This article documents the addition of 277 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Ascochyta rabiei, Cambarellus chapalanus, Chionodraco hamatus, Coptis omeiensis, Cynoscion nebulosus, Daphnia magna, Gerbillus nigeriae, Isurus oxyrinchus, Lates calcarifer, Metacarcinus magister, Oplegnathus fasciatus, Pachycondyla verenae, Phaethon lepturus, Pimelodus grosskopfii, Rotylenchulus reniformis, Scomberomorus niphonius, Sepia esculenta, Terapon jarbua, Teratosphaeria cryptica and Thunnus obesus. These loci were cross-tested on the following species: Austropotamobius italicus, Cambarellus montezumae, Cambarellus puer, Cambarellus shufeldtii, Cambarellus texanus, Chionodraco myersi, Chionodraco rastrospinosus, Coptis chinensis, Coptis chinensis var.

[Characterization of the genetic diversity of the fish Brycon henni (Characiformes: Characidae) in central Colombia with RAPD markers].

Knowledge on the genetic diversity of wild fish species is essential for conservation and appropriate management of individuals in repopulation programs. In Colombia, Brycon henni has been reported in the Magdalena and Cauca river basins, but the population and range have diminished as a consequence of anthropic activities. In this study, the Random Amplified Polymorphic DNA (RAPD) was used to estimate the actual genetic structure in this species.

Endogenously generated hydrogen peroxide induces apoptosis via mitochondrial damage independent of NF-kappaB and p53 activation in bovine embryos.

Hydrogen peroxide (H(2)O(2)) has been implicated as a key molecule in arresting embryonic development; however, its mechanism of action is not fully established. The aim of the present study was to determine the chronological generation of H(2)O(2) from oocyte to morula, and to examine the relationship of H(2)O(2) with loss of mitochondrial membrane potential, nuclear factor kappa-B (NF-kappaB), p53, caspase-3 activation, and cell death in bovine embryos in vitro. Accordingly, superoxide anion radicals were detected between 32 and 120 h after in vitro fertilization, but higher percentages of oxygen radicals were found in non-competent embryos (n=73, 22 to 34%) than in competent embryos (n=73, 0 to 1%; P<0.

[Evaluation of the calcium ionophore induced acrosome reaction: a more realistic approach to the fecundant capacity of the spermatozoid].

Objectives: The aim of this investigation was to evaluate the acrosome reaction by fluorescence microscopy and the flow citometry in a group of men with unknown fertility.

Methods: Each individual gave a semen sample for the analysis of the calcium ionophore-induced acrosome reaction after the capacitation process, using lectin Pisum sativum aglutinina (fluorescence microscopy) and antibody anti-CD46 (flow citometry).

Results: Five of out six individuals increased the number of the sperm with calcium ionophore-induced acrosome reaction versus the percentage of spontaneous acrosome reaction, using fluorescence microscopy (7.

[Sperm parameters in 113 subjects after recent fatherhood did not correlate with WHO standards].

Objectives: This is a descriptive prospective study of semen analysis in 113 men who were candidates for vasectomy as an option for fertility control after a recent event of fertility.

Methods: Each subject provided a semen sample for the analysis of volume, pH, spermatozoid concentration, mobility, and morphology. The results were compared with the reference values established by WHO.

Abundant mtDNA diversity and ancestral admixture in Colombian criollo cattle (Bos taurus).

Various cattle populations in the Americas (known as criollo breeds) have an origin in some of the first livestock introduced to the continent early in the colonial period (16th and 17th centuries). These cattle constitute a potentially important genetic reserve as they are well adapted to local environments and show considerable variation in phenotype. To examine the genetic ancestry and diversity of Colombian criollo we obtained mitochondrial DNA control region sequence information for 110 individuals from seven breeds.