Development of a rapid sanitization solution for silica-based protein A affinity adsorbents.

Protein A chromatography media require sanitization between batches as well as prior to long-term storage. While sodium hydroxide (NaOH) is probably one of the most widely used sanitants within the bioprocess industry, it cannot be used with silica- or controlled pore glass (CPG)-based adsorbents due to the instability of the base matrix at high pH. Benzyl alcohol is commonly used for sanitizing such adsorbents, though extended contact times may be required to meet desired microbial log reduction values, especially for fungal and bacterial spore formers.

Alteplase as a catheter locking solution: in vitro evaluation of biochemical stability and antimicrobial properties.

Purpose: To reduce potential complications of fibrin deposition to catheter surfaces, there is increasing empiric use of alteplase as a catheter lock solution. The purpose is to evaluate the properties of alteplase when reconstituted in sterile water (SW) or bacteriostatic water (BW) for prolonged periods.

Materials And Methods: Alteplase in glass vials was reconstituted (1 mg/mL) with SW or BW (0.