Detection of synergistic combinations of Baccharis extracts with terbinafine against Trichophyton rubrum with high throughput screening synergy assay (HTSS) followed by 3D graphs. Behavior of some of their components.

Forty four extracts from nine Baccharis spp. from the Caulopterae section were tested in combination with terbinafine against Trichophyton rubrum with the HTSS assay at six different ratios with the aim of detecting those mixtures that produced a ≥50% statistically significant enhancement of growth inhibition. Since an enhanced effect of a combination respective of its components, does not necessarily indicate synergism, three-dimensional (3D) dose-response surfaces were constructed for each selected pair of extract/antifungal drug with the aid of CombiTool software.

Isolation of antifungal saponins from Phytolacca tetramera, an Argentinean species in critic risk.

The methanolic extract of the berries of Phytolacca tetramera, an Argentinean species submitted to a great anthropic impact, and therefore in critic risk of extinction, not previously studied, showed antifungal activity against opportunistic pathogenic fungi. Through fractionation of the extract followed by agar dilution assays, three monodesmosidic triterpenoid saponins have been isolated from the butanolic extract of P. tetramera.