Real-Time FRET-Based Measurements of Ca/PKA Dynamics in Plasma Membrane Microdomains of Primary Hippocampal Neurons.

Both Ca and protein kinase A (PKA) are multifaceted and ubiquitous signaling molecules, essential for regulating the intricate network of signaling pathways. However, their dynamics within specialized membrane regions are still not well characterized. By using genetically encoded fluorescent indicators specifically targeted to distinct plasma membrane microdomains, we have established a protocol that permits observing Ca/PKA dynamics in discrete neuronal microdomains with high spatial and temporal resolution.