Publications by authors named "Marta S Pierdominici"
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
View Article and Find Full Text PDFMechanisms leading to low platelet count in immune thrombocytopenia (ITP) involves both decreased production and increased destruction of platelet. However, the contribution of these pathologic mechanisms to clinical outcome of individual patients is uncertain. Here we evaluated different pathogenic mechanisms including in vitro megakaryopoiesis, platelet/megakaryocyte (MK) desialylation and MK apoptosis, and compared these effects with thrombopoyesis and platelet apoptosis in the same cohort of ITP patients.
View Article and Find Full Text PDFMechanisms leading to decreased platelet count in immune thrombocytopenia (ITP) are heterogeneous. This study describes increased platelet apoptosis involving loss of mitochondrial membrane potential (ΔΨm), caspase 3 activation (aCasp3) and phosphatidylserine (PS) externalization in a cohort of adult ITP patients. Apoptosis was not related to platelet activation, as PAC-1 binding, P-selectin exposure and GPIb-IX internalization were not increased.
View Article and Find Full Text PDFArticle Synopsis
- The study aims to understand the reasons behind low platelet counts in immune thrombocytopenia (ITP) by examining how ITP plasma affects proplatelet formation (PPF) in healthy megakaryocytes.
- Patient plasma was found to inhibit PPF in a dose-dependent manner, altering the structure of proplatelets, but increased megakaryocyte apoptosis was not responsible for this impairment.
- The research indicates that auto-antibodies in ITP plasma disrupt normal thrombopoiesis, particularly through their effects on integrins crucial for platelet formation, highlighting new insights into the mechanisms behind platelet count reduction in ITP.