Publications by authors named "Marta Pedziwiatr"

In this study, we tested the possibility of creating complexes of two proteins by fusing them with heterodimerizing helices. We used the fluorescent proteins GFP and mCHERRY expressed with a His-tag as our model system. We added heterodimer-forming sequences at the C- or N- termini of the proteins, opposite to the His-tag position.

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Article Synopsis
  • Quantum dots (QDs), useful as fluorescent labels, face challenges in bioscience applications due to surface instability and hydrophobicity.
  • This study compared two protein types - Pumilio homology domain (Puf) and leucine-rich repeat (LRR) - as potential covers for spherical QDs made of cadmium selenide, ultimately finding Puf to be more effective.
  • The research demonstrated that the Puf protein maintains its structure and does not lead to harmful reactive oxygen species when in contact with QDs, marking progress in developing a stable, biocompatible QD cover.
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Solvated tris-complexes of (R)- and (S)-1,1'-binaphthyl-2,2'-dyil phosphate with lanthanum(III) and europium(III) centers were prepared and characterized by spectroscopic methods and elemental analysis. Circularly polarized luminescence (CPL) spectra for the optically active isomers of the europium complexes are also reported.

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