Profiling of high molecular weight esters by flow injection analysis-high resolution mass spectrometry for the characterization of raw and archaeological beeswax and resinous substances.

This work presents a method to characterize high molecular esters in beeswax and resinous substances based on the use of microwave-assisted extraction and flow injection analysis-high resolution mass spectrometry that combines the high efficiency of the extraction procedure with the advantages of high resolution mass spectrometry. This approach allows us to identify archaeological beeswax and plant resinous substances by the characterization of the survived intact high molecular weight components. By this way, several raw materials (beeswax, pine resin and pitch, and resin extracted from Euphorbia tirucalli) were studied and used as reference substances.