[Recombinant granulocyte-colony stimulating factor (filgrastim): optimization of conditions of isolation and purification from inclusion body].

We developed a unified process platform for two recombinant human GCSF medicines--one with the non-prolonged and the other with prolonged action. This unified technology led to a simpler and cheaper production while introduction of the additional pegylation stage to the technological line eased obtaining of the medicines with different action and allowed to standardize technological process documenting according to GMP requirements.

[Recombinant granulocyte-colony stimulating factor (filgrastim): optimization of conjugation with polyethylene glycol].

In order to create an active pharmaceutical substance of the drug with prolonged action the modification of recombinant human granulocyte colony-stimulating factor GCSF (filgrastim) with polyethylene glycol (PEG, M 21.5 kDa) was conducted. A method for preparation of PEG-filgrastim designed for the development and scaling-up of the technological process of production was described.

[Radiological and hygienic approaches to solving the problem of environmental safety of radioactive waste storages].

The paper presents general approaches to solving the problems associated with the radioecological safety of radioactive waste (RAW) storages. It considers the influence of climatic factors on the possible release of radionuclides into the environment. The authors have made as follows: analysis of the significance of main scenarios for radionuclide release into the environment and the natural and climatic conditions of the existing areas of near-surface RAW storages in the Russian Federation; conditional zoning of the Russian Federation according to the balance of atmospheric precipitation.

[On quality grade of geologic barriers in construction and running the near-surface depots for radioactive waste with low and medium activity].

The article contains data on changed state of natural (geologic) barrier in construction and running the depots for radioactive waste. The authors stress over 10-fold increased water-permeability of upper part of radioactive waste placement ground, formation of antropogenous (technogenic) water-bearing level and changed filtration flow direction. Based on radionuclides migration modelling, the authors determined possibility of more intensive relocation of radionuclides in subsoil.

[Clinical trials of the new skin test Diaskintest for the diagnosis of tuberculosis].

A new reagent for a skin test given the name Diaskintest has been designed for the screening diagnosis of tuberculosis and preclinical and clinical trials conducted. Preclinical trials were carried out on 315 laboratory animals (guinea-pigs, albino mice). The reagent Diaskintest was ascertained to be nontoxic, to have no sensitizing properties, to be safe and specific, and to induce no positive reactions in BCG-vaccinated animals and healthy guinea-pigs.