Femoral medullary infarction prevalence with the Zurich Cementless Canine Total Hip arthroplasty.

Objective: To document the prevalence of femoral medullary infarction associated with the Zurich Cementless Total Hip Replacement (ZCTHR) system in dogs.

Study Design: Case series.

Animals: Dogs (n=35) with 41 ZCTHR implants.

Kinematic analysis of the hind limb during swimming and walking in healthy dogs and dogs with surgically corrected cranial cruciate ligament rupture.

Objective: To determine hip, stifle, and tarsal joint ranges of motion (ROM) and angular velocities during swimming and walking in healthy dogs and dogs with surgically corrected cranial cruciate ligament (CCL) rupture.

Design: Prospective clinical study.

Animals: 13 healthy dogs and 7 dogs with CCL rupture.

Effects of postoperative rehabilitation on limb function after cranial cruciate ligament repair in dogs.

Objective: To determine the effects of early postoperative rehabilitation on limb function in dogs after surgery for ruptured cranial cruciate ligament (RCCL).

Design: Prospective clinical study.

Animals: 51 client-owned dogs.

Seroepidemiologic study of Neospora caninum in dairy herds.

Objective: To identify risk factors associated with Neospora caninum infection in dairy herds in Québec and to estimate seroprevalence in case and control herds.

Design: Herd-based case-control and seroprevalence study.

Animals: 3,059 cows from 24 case and 22 control dairy herds in Québec.

Importance of primer selection in the application of PCR technology to the diagnosis of bovine leukemia virus.

The polymerase chain reaction (PCR) was used to detect bovine leukemia virus in bovine blood samples. When applied to leucocytes extracted from the blood samples, the standard method of DNA extraction gave good correlation with agar gel immunodiffusion, but a method in which 5 microliters of blood was the starting material was unreliable. Selection of the primers was important, and differences in results were observed when the PCR method was applied to blood samples from different geographic areas.

Detection of bovine coronavirus and type A rotavirus in neonatal calf diarrhea and winter dysentery of cattle in Quebec: evaluation of three diagnostic methods.

The use of direct electron microscopy, enzyme-linked immunosorbent assay, and protein A-gold immunoelectron microscopy for the identification of bovine coronavirus and type A rotavirus were examined. Two hundred and forty-nine samples from diarrheic calves and winter dysenteric cattle from seven geographic areas in Quebec were examined for the presence of viruses by direct electron microscopy of negatively stained preparations. In addition, all the samples were analyzed by enzyme-linked immunosorbent assay, and a random selection of 47 samples were also analyzed by protein A-gold immunoelectron microscopy.

Early detection of bovine leukemia virus in cattle by use of the polymerase chain reaction.

A study was performed to determine whether experimentally induced bovine leukemia virus (BLV) infection in cattle could be detected earlier by use of polymerase chain reaction (PCR) amplification of genomic DNA extracted from leukocytes than by use of conventional agar-gel immunodiffusion (AGID). The PCR primers were designed to amplify a 375-base-pair region of the proviral gag gene. Five cows were identified that were BLV-negative on the basis of AGID and PCR results.

Bovine viral diarrhea virus-infected MDBK monolayer as antigen in enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies in bovine sera.

A specific ELISA for the detection of IgG antibodies against bovine viral diarrhea virus (BVDV) has been developed and was compared with the seroneutralization (SN) titers for a total of 60 bovine serum samples. A BVDV-infected Madin-Darby Bovine Kidney (MDBK) cell monolayer served as test antigen. The following results were obtained: a coefficient of correlation (r) of 0.

Coronaviruses associated with outbreaks of transmissible enteritis of turkeys in Quebec: hemagglutination properties and cell cultivation.

Coronaviruses were observed by electron microscopy in the intestinal contents of turkeys in Quebec flocks where repeated outbreaks of enteritis occurred. Three isolates could be serially propagated in turkey embryos inoculated by the amniotic route with clarified intestinal contents. Purification and concentration of viral particles contained in intestinal contents of infected embryos were achieved by precipitation with polyethylene glycol and ultracentrifugation on sucrose density gradients.

[The debate within the CSN on the organization of mental health.].

The authors attempt in this article, to account for the political stakes in mental health which are in process of elaboration, among the C.S.N.

Papovavirus induced feather abnormalities and skin lesions in the budgerigar: clinical and pathological findings.

Feather abnormalities and skin lesions caused by a papovavirus infection in budgerigars are described. Diseased one to 15 day old birds displayed a lack of nestling down feathers and filoplumes on the head and neck. Survivors older than 15 days exhibited retarded growth and temporary absence of feathers variable from bird to bird.

Interspecific differences between the DNA restriction profiles of canine adenoviruses.

Identification of canine adenovirus-1 ( CAV -1) and canine adenovirus-2 ( CAV -2) strains was done by electrophoresis of restriction endonuclease-fragmented viral DNA. Results obtained with this sensitive and reproducible technique clearly show that CAV -2 is not a variant of CAV -1 but a distinct virus.

Unambiguous typing of canine adenovirus isolates by deoxyribonucleic acid restriction-endonuclease analysis.

Viral deoxyribonucleic acid extracted from a limited number of cells infected with canine adenovirus type 1 or type 2 was cleaved with several restriction endonucleases. Agarose gel electrophoresis of the limit digests showed stable differences between the canine adenovirus type 1 and type 2 cleavage patterns. Rapid and accurate typing of large numbers of clinical isolates may thus be done by deoxyribonucleic acid restriction-endonuclease analysis.

Serology of bovine parainfluenza virus type 3: comparison of the enzyme linked immunosorbent assay and hemagglutination inhibition.

An enzyme linked immunosorbent assay (ELISA) for the detection of antibody to bovine parainfluenza virus type 3 has been compared with the hemagglutination inhibition test on 130 field sera, and seven other paired sera showing a significant raise of titers. The ELISA was found to be four to 64 times more sensitive than the hemagglutination inhibition test and the two tests demonstrated a good correlation.

Types of avian infectious bronchitis strains isolated in Quebec.

Between 1976 and 1980, 24 isolates of infectious bronchitis virus were obtained from Quebec flocks. The serological classification of these isolates was demonstrated by cross neutralization tests using antisera to 13 different reference virus strains. Of the 24 isolates, ten were identified as Connecticut, six Holland and one SE-17 types.

A generalized inclusion body disease in the budgerigar (Melopsittacus undulatus) caused by a papovavirus-like agent.

High mortality rates have been reported in budgerigars between one and 15 days of age in 19 aviaries in the Province of Quebec. The most consistent signs of disease were abdominal distention, lack of down feathers on the back and abdomen, lack of filoplumes on the head and neck, and retarded growth of the tail and contour feathers in birds that either survived or died later. Internal gross lesions were hydropericardium, enlarged heart and liver with multiple pinpoint white spots or large, yellow foci, pale or congested kidneys, congested lungs, and ascites.

[Diagnosis of septic abortion in dairy cows].

During a two year period, March 1977 to April 1979, a total of 92 bovine abortions were studied. The cause of abortion was determined in 34.8% of the cases examined.

Inner structures of some coronaviruses.

When treated with formaldehyde, Tween 80, sodium oleate and Nonidet P-40, avian infectious bronchitis virus, porcine transmissible gastroenteritis virus, neonatal calf diarrhea coronavirus, porcine hemagglutinating encephalomyelitis virus as well as the human coronavirus show similar inner structures by negative staining. The first one is an inner membranous bag. This structure could be evaginated following treatments used and does not show the characteristic projections of coronaviruses.

Preliminary report on the observation of a coronavirus in the intestine of the laboratory rabbit.

Intestinal infections are a major cause of mortality in domestic rabbits. Although a few pathogenic organisms have been associated with this type of infection, it is often impossible to demonstrate the presence of any etiologic agents. Recently viral particles were observed in the intestine of diseased rabbits submitted to our laboratory for diagnosis.

Bovine respiratory syncytial virus in Quebec: antibody prevalence and disease outbreak.

The prevalence of antibody to bovine respiratory syncytial virus in Quebec and the role of the virus in a respiratory disease outbreak was investigated. The indirect immunofluorescent, neutralization and haemagglutination inhibition techniques were used to carry out this study. Of the 1,444 adult animals examined 519 (35.

Diagnosis of rotavirus, adenovirus, and herpesvirus infections by immune electron microscopy using a serum-in-agar diffusion method.

The sensitivity of immune electron microscopy (IEM) for the detection and identification of bovine rotavirus, infectious bovine rhinotracheitis virus (IBR), and canine adenovirus has been studied by using the serum-in-agar (SIA) method in which a specific antiserum has been incorporated in agar.

[Isolation of infectious bovine rhinotracheitis virus from the tarsal joint of a bull (author's transl)].

Isolation Of Infectious Bovine Rhinotracheitis Virus From The Tarsal Joint Of A Bull: Infectious bovine rhinotracheitis virus was isolated from the right tarsal joint of a bull who was showing signs of a systemic viral infection. The clinical signs manifested by 16 bulls of this herd are described, the laboratory methods used are listed and the results are analysed and discussed.

Use of electron microscopy and an enzyme-linked immunosorbent assay for the detection of rotaviruses in neonatal calf diarrhea.

Electron microscopy and an enzyme-linked immunosorbent assay were compared for the diagnosis of rotaviruses associated with neonatal calf diarrhea. One hundred percent correlation was observed when 125 samples were tested by both techniques. Both techniques were equally efficient in detecting rotaviruses.