Papillary necrosis in experimental renal transplantation in the rat.

Renal papillary necrosis is a frequent complication of unsuccessful renal transplantation in rats, occurring in both isografts and allografts. Papillary necrosis does not occur alone, but only and inevitably in association with severe cortical damage. The pattern of the lesion is different from other forms of papillary necrosis in that the least severe lesions occur in the outer medulla and the more severe lesions involve both medulla and papilla.

Augmentation of serum bactericidal activity by paldimycin.

At concentrations below the MIC, paldimycin induced changes in Staphylococcus aureus 502A (UC 9116, ATCC 28417) which increased its sensitivity to serum. The enhanced sensitivity to serum was concentration dependent with the maximal sensitivity found when bacteria were grown in approximately 1/10 MIC of paldimycin. Within an 1-hour incubation, S.

Deep white matter infarction: correlation of MR imaging and histopathologic findings.

Focal and confluent areas of periventricular hyperintensity have been reported on magnetic resonance (MR) images in 30% of patients over 60 years of age. In order to better understand the pathologic basis of these lesions, the authors studied 14 formalin-fixed brains with MR imaging. Multiple focal areas of hyperintensity were identified in the periventricular white matter in three of the 14 brains studied (21%).

Multiple sclerosis is a chronic central nervous system infection by a spirochetal agent.

Multiple Sclerosis (MS) is a chronic central nervous system (CNS) infection similar to Lyme Disease or Neurosyphilis in its latency period, pathogenesis, symptoms, histopathology and chronic CNS involvement. It does not have as yet a fully identified spirochetal etiological agent. Much research and clinical support for this hypothesis was published before 1954 and is based on silver staining of neural lesions, animal isolation of the etiologic agent and the characteristic symptoms and pathogenesis of the disease.

The thigh flap: an osteomyocutaneous free-flap model in the rat.

A new experimental model for free-flap transfer has been developed in the rat. This "thigh flap" is an osteomyocutaneous free flap of bone (femur), muscle (thigh), and skin (groin) based on the femoral vessels. The flap is harvested from the left groin and thigh of an inbred female rat and is transferred to a subcutaneous pocket in the left groin of a male rat of the same inbred strain.

Prevalence of depression and distress in a large sample of Canadian residents, interns, and fellows.

Using data from 1,805 interns, residents, and fellows in Ontario, Canada, the authors report the prevalence of symptoms measured by the Center for Epidemiologic Studies Depression Scale (CES-D). They found that the proportion of subjects scoring as depressed was somewhat higher than that found in community studies. Women had higher depression scores than men.

Urinary risk factors in calcium oxalate stone disease: comparison of men and women.

The daily excretion of calcium, oxalate, uric acid and glycosaminoglycans, the 24-h urinary pH and volume, and the inhibitory effects of the urines on calcium oxalate crystal growth and aggregation, were measured in 44 normal women, 41 normal men, 32 female stone formers and 63 male stone formers. No significant differences could be found between the normal men and women, the male and female stone formers, or between the patients and their normal controls with regard to the excretion of oxalate and glycosaminoglycans, and the urinary pH. The normal women exhibited significantly lower urinary volumes and excreted less calcium per day than did the other subject groups.

Macromolecules inhibit calcium oxalate crystal growth and aggregation in whole human urine.

Removal of macromolecules with Mr greater than 10,000 had no discernible effect on the detectable nucleation of calcium oxalate crystals from undiluted human urine, but promoted the deposition of crystalline material and markedly increased the degree of aggregation of the precipitated crystals. Calcium oxalate crystals and crystal aggregates precipitated from ultrafiltered urine were, on average, 68% larger than those deposited from whole urine. These findings suggest that urinary macromolecules play a key role in preventing calcium oxalate kidney stone disease by inhibiting the formation of large crystal aggregates and thereby reducing the probability of particle retention in the kidney tubules.

Paulomycin-related antibiotics: paldimycins and antibiotics 273a2. Isolation and characterization.

The isolation of paulomycins A and B from fermentations of Streptomyces paulus has been reported earlier [J. Antibiotics 35: 285-294, 1982]. Further work on the antibiotics produced by S.

Sexual dysfunction after prostatectomy.

One of the major and disconcerting complications of prostatectomy is impotence. Several studies have reported only a small reduction in potency rates following prostatectomy but have not examined the effect of surgery per se on a group of men in whom one could expect a significant incidence of reduced potency. In order to rectify this defect, a prospective study was undertaken to assess the effect of transurethral resection of the prostate (TURP) and other general surgical procedures on two age-matched populations.

Enzymatic phosphorylation of macrolide antibiotics.

Five macrolide antibiotics (erythromycin A, 1; oleandomycin, 3a; tylosin, 4a; spiramycins, 5a; leucomycin A3, 6a) have been phosphorylated enzymatically using cell-free extracts derived from Streptomyces coelicolor UC 5240. The necessary cofactors and the rates of the conversion have been determined.

Hepatitis B and renal transplantation.

Renal transplantation in HBsAg+ chronic carriers has a relative low risk of progressive liver disease, with mortality associated with liver disease at 7%. In contrast, HBsAg+ recipients who acquired their disease in the early posttransplant period had a mortality of 60%. HBeAg-positive patients who remain persistently positive are a subgroup with a poor prognosis and should not be offered a renal transplant.

Rat pancreas preservation for transplantation.

This study has shown that phosphate-buffered sucrose is a suitable preserving solution for the cold storage of rat pancreata for 24 hours. Isotonic citrate does not appear to be as effective a preserving solution under these conditions. Preservation of endocrine function is evident, but preservation of exocrine function is effected by obstruction or occlusion of the ductocystostomy, which may occur some time after transplantation and may not be related to transplant conditions.

Effects of ageing and hormonal manipulations on the level of oestrogen receptors in the guinea-pig prostate.

Cytosolic oestrogen receptor levels in guinea-pig prostate tissue were found to decrease with increasing age, irrespective of whether the binding was expressed relative to cytosolic protein or cellular DNA. This decrease in oestrogen receptor levels was also observed using enriched fibromuscular stromal tissue prepared by mechanical fractionation of the prostate. The most pronounced change in cytosolic oestrogen receptor levels (from 133 to 35 fmol/mg protein) occurred at the onset of puberty.

The effect of serum on the crystallization of calcium oxalate in whole human urine: inhibition disguised as apparent promotion.

The effect of serum on calcium oxalate crystallization was studied in whole human urine. At concentrations between 0.005% to 0.

Glomerular epithelial cell lesions in rat renal isografts.

Visceral glomerular epithelial cell lesions--microvillus formation, loss of foot processes, osmiophilic inclusion droplets, balloon-like malformation of cell processes, degeneration, necrosis, and loss of cell processes from capillary basement membranes--are found in rat renal isografts 1 mth after transplantation. The lesions, which are most readily recognized in perfusion-fixed material, are essentially focal, affecting neither all glomeruli, nor all cells in any glomerulus, bear no relation to the degree of interstitial nephritis in the graft, and are associated with albuminuria and with focal capillary sclerosis in some glomeruli. They are not restricted to renal isografts but are found in aging rats, in different experimental models of glomerular disease and in clinical glomerular disorders, again in association with proteinuria and glomerulosclerosis.

Influence of the lactoperoxidase system on susceptibility of the udder to Streptococcus uberis infection.

Lactoperoxidase (LP), thiocyanate (SCN-), pH and somatic cell counts (SCC) were measured in mammary secretions from 20 cows collected 14 d before drying-off, 7 and 21 d after drying-off, and 3-18 d postcalving. The inhibitory activity of the secretions on Streptococcus uberis was determined and the susceptibility of the udder to infection by this organism was tested by intramammary infusion of 250 colony forming units at the above stages. LP, SCN-, pH and SCC increased during involution and fell postcalving.

An improved method for the preparation and culture of urothelial cells.

Urothelial cells have been prepared by a new method involving collagenase treatment of the lumen of a ureter. These cells have been identified as epithelial and successfully subcultured. In addition, we have observed that growth rate is significantly increased by the inclusion of an extract of bovine hypothalamus in the growth medium.

Influence of mastitis on growth of starter organisms used for the manufacture of fermented milks.

The effect of milk containing increased somatic cells on the starter organisms Streptococcus thermophilus, Lactobacillus bulgaricus and L. acidophilus was examined. Increased somatic cell count resulted in stimulation of Str.

The assessment of urinary catheter toxicity using cell cultures: validation by comparison with an animal model.

A cell culture technique for assessing the toxicity of urinary catheters is described. The inhibitory effect of catheter extracts on the uptake of 3H-labelled thymidine by L929 mouse fibroblasts and human prostate derived epithelial cells in culture was measured. The concentration of extract which depressed uptake to 50 per cent of control (IC50) was determined for catheters of different composition.

Glomerular damage after kidney preservation.

Severe proteinuria occurs during isolated organ perfusion of kidneys removed from SD and DA rats and subjected to 24-hr cold preservation. In both strains increased glomerular permeability was associated with changes in glomerular visceral epithelial cells, particularly cytoplasmic edema and detachment of cells from capillary basement membranes. Foot processes were intact and staining for sialoglycoprotein was retained.