Assessment of Covid-19 vaccine confidence among healthcare personnel in the safety-net sector in the United States and Puerto Rico.

Background: This study aimed to assess COVID-19 vaccine confidence among healthcare personnel in the safety net sector of the United States and Puerto Rico. This study aimed to examine the extent to which increased knowledge and positive attitudes toward COVID-19 vaccine safety and efficacy were associated with healthcare workers' COVID-19 vaccination status and their recommendation of the vaccine to all patients.

Methods: Online survey data were collected from health care workers working in Free and Charitable Clinics across the United States and Federally Qualified Health Centers in Puerto Rico.

Neutrophilia with damage to the blood-brain barrier and neurovascular unit following acute lung injury.

Background: Links between acute lung injury (ALI), infectious disease, and neurological outcomes have been frequently discussed over the past few years, especially due to the COVID-19 pandemic. Yet, much of the cross-communication between organs, particularly the lung and the brain, has been understudied. Here, we have focused on the role of neutrophils in driving changes to the brain endothelium with ensuing microglial activation and neuronal loss in a model of ALI.

Neutrophilia with damage to the blood-brain barrier and neurovascular unit following acute lung injury.

Background: Links between acute lung injury (ALI), infectious disease, and neurological outcomes have been frequently discussed over the past few years, especially due to the COVID-19 pandemic. Yet, much of the cross-communication between organs, particularly the lung and the brain, has been understudied. Here, we have focused on the role of neutrophils in driving changes to the brain endothelium with ensuing microglial activation and neuronal loss in a model of ALI.

Unraveling the immune and metabolic changes associated with metritis in dairy cows.

The objective was to unravel the peripartum immune and metabolic changes associated with metritis in Holstein cows. Holstein cows (n = 128) had blood collected at -14, 0, 3, and 7 d relative to parturition (DRP). Flow cytometry was used to evaluate blood leukocyte counts, proportions, and activation.

[Leadless pacemaker implantation. Case report].

We report 67-year-old woman with a sinus node dysfunction and diffuse conduction system disease, with a history of recurrent paroxysmal atrial fibrillation. She was admitted to the Hospital due to palpitations, dizziness, and vertigo, attributing the symptoms to the rhythm disorder described, for which the implantation of a pacemaker was indicated. With a history of tracheal cancer treated with radio and chemotherapy, and chronic steroid therapy for rheumatoid arthritis, she had an important limitation in vascular access for a conventional pacemaker, so, added to a high risk of infection, a decision was made to implant a leadless pacemaker.

Dietary LC-PUFA and environmental salinity modulate the fatty acid biosynthesis capacity of the euryhaline teleost thicklip grey mullet (Chelon labrosus).

The capacity to biosynthesise long-chain (≥C20) polyunsaturated fatty acids (LC-PUFA) depends upon the complement and function of key enzymes commonly known as fatty acyl desaturases and elongases. The presence of a Δ5/Δ6 desaturase enabling the biosynthesis of docosahexaenoic acid (22:6n-3, DHA) through the "Sprecher pathway" has been reported in Chelon labrosus. Research in other teleosts have demonstrated that LC-PUFA biosynthesis can be modulated by diet and ambient salinity.

Valorization of : From Invasive Plant to Fiber Reinforcement of Injected Composites.

During the control campaigns of (invasive species widespread worldwide), the generated waste has accumulated in landfills. This study investigates its use to obtain fibers for their application as reinforcement of polymeric materials for injection molding, thus facilitating and promoting alternatives for the long-term sustainable management of . The extracted fibers were treated with alkaline, silane, acetic acid, and combined alkaline and silane treatments.

Valorization of Seaweed Wracks: Inclusion as Additive in Diets for Grass Carp ().

Macroalgae have been recently described as a potential ingredient for aquafeeds, exerting several physiological benefits. Grass carp () is a freshwater species, which has been the major fish species produced in the world in the last years. In order to determine the potential use of macroalgal wracks in fish feeding, juveniles were fed with an extruded commercial diet (CD) or the CD supplemented with 7% of a wind dried-powder (1 mm) from either a multispecific macroalgal wrack (CD + MU7) or a monospecific macroalgal wrack (CD + MO7) obtained from Gran Canaria island (Spain) coasts.

Early-life heat stress exposure impacts dairy calf feeding and thermoregulatory behavior.

Heat stress has well-known influences on dairy calf physiology, but less is understood about calf behavioral responses to heat stress. Herein, we evaluated milk replacer intake, standing activity, and lying behaviors of calves exposed to prenatal or postnatal heat stress or both. Holstein calves were born to dams experiencing heat stress (HT; shade of a freestall barn) or cooling (CL; shade, fans, and soakers) during late gestation [~44 d before calving, prenatal; mean daily temperature-humidity index (THI) = 78].

Culture of endometrial epithelial cells collected by a cytological brush in vivo.

In cattle, mechanistic studies of endometrial function rely on cell lines or primary culture of cells harvested postmortem. Understanding the endometrial physiology in dairy cows is essential, because approximately 50% of pregnancies are lost in the first 3 wk of gestation for unknown reasons. The objective was to validate an in vivo, minimally invasive, and estrous cycle stage-specific method to obtain endometrial luminal epithelial cells for culture.

Evaluation of water footprint of urban renewal projects. Case study in Seville, Andalusia.

This publication presents a methodology for the evaluation of the water footprint of urban renewal projects. The indirect water footprint is obtained by adding together the embodied or virtual water of the materials incorporated in the 12-month project, while the direct footprint is mainly due to the green areas and rainwater collection system over its 40-year life span. The methodology, originally defined for the agricultural sector, is adapted to an urban system that includes gardens and sustainable urban drainage systems.

Metabolic and molecular evidence for long-chain PUFA biosynthesis capacity in the grass carp Ctenopharyngodon idella.

There is a growing interest to understand the capacity of farmed fish species to biosynthesise the physiologically important long-chain (≥C) n-3 and n-6 polyunsaturated fatty acids (LC-PUFAs), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (ARA), from their C PUFA precursors available in the diet. In fish, the LC-PUFA biosynthesis pathways involve sequential desaturation and elongation reactions from α-linolenic acid (ALA) and linoleic acid (LA), catalysed by fatty acyl desaturases (Fads) and elongation of very long-chain fatty acids (Elovl) proteins. Our current understanding of the grass carp (Ctenopharyngodon idella) LC-PUFA biosynthetic capacity is limited despite representing the most farmed finfish produced worldwide.

Responding to a school SARS-CoV-2 outbreak: Insights and policy implications emerging from the pandemic.

Background: The SARS-CoV-2 pandemic has affected also the school environment. Prolonged closures and the weakness of available data prevent a definitive answer to the question of school transmission. We report our experience of responding to COVID-19 cases in the school setting, presenting a case study of the management of an outbreak in a large school.

Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes.

Many aspects of the bovine immune system remain poorly characterized, which poses an obstacle to improving dairy cow health. Herein, we describe two flow cytometry panels that included antibodies against CD8α, CD4, TCR-δ, CD172α, CD14, MHCII, CD21, CD62L, and CD11b. These panels were used to characterize the phenotype of leukocyte subpopulations from the peripheral blood of 30-day old Holstein calves and Holstein cows at 260 d of gestation and calving.

An annual study on plastic accumulation in surface water and sediment cores from the coastline of Tenerife (Canary Island, Spain).

Sediment core samples from high tide lines and in submerged zones as well as surface water samples from eight beaches of Tenerife were analysed. Sampling was conducted over a period of one year in intervals of 5 weeks. The majority of particles were found in the high tide sediment (66%), followed by water samples (23%) and finally in sediment from submerged zones (11%).

Valorization of Invasive Plants from Macaronesia as Filler Materials in the Production of Natural Fiber Composites by Rotational Molding.

This paper compares the mechanical properties of different natural fiber composites produced by rotational molding as a way of waste valorization from campaigns to control invasive plant species in Macaronesia. Rotomolded parts produced with polymeric matrices (polyethylene) and filled with up to 20% by weight of cellulosic fibers obtained from L., , and plants were characterized in terms of tensile, flexural, and impact strength.

spp. Fibre Characterisation to Obtain Sustainable Materials in the Composites Field.

Some studies have evaluated the use of as reinforcement for polymeric matrices, obtaining good results in energy absorption tests and increasing the tensile elastic modulus. However, no studies focusing on the previous characterisation of the fibres and their treatment to improve compatibility with polymeric matrices have been found. This work analyses the chemical composition of (OM) and (OD) cladodes and fibre, studying how different treatments influence it.

Environmental Hazards of Giant Reed ( L.) in the Macaronesia Region and Its Characterisation as a Potential Source for the Production of Natural Fibre Composites.

This paper summarises the results obtained from the characterisation of giant reed ( L.) plant and fibres. The research is part of a project developed in the Macaronesia region, of which the aim is to demonstrate the feasibility of using biomass from invasive plant species in the composites sector as a way of financing control campaigns and habitats conservation labours.

Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves.

Exposure to heat stress can alter the development and immune system function in dairy calves. Serotonin is an immunomodulatory biogenic amine that functions as a neurotransmitter and as a stress-response mediator. Our objectives were to characterize the patterns of serum serotonin concentrations and the pattern of serotonin-related genes expressed by immune cells of calves exposed to chronic heat stress or heat stress abatement during early life, and to explore whether these might relate to immune system development.

Combined Manufacturing Process of Copper Electrodes for Micro Texturing Applications (AMSME).

Surface texturing has brought significant improvements in the functional properties of parts and components. Sinker electro discharge machining (SEDM) is one of the processes which generates great texturing results at different scale. An electrode is needed to reproduce the geometry to be textured.

A first-in-class, first-in-human, phase I trial of CIGB-552, a synthetic peptide targeting COMMD1 to inhibit the oncogenic activity of NF-κB in patients with advanced solid tumors.

CIGB-552 is a synthetic peptide that interacts with COMMD1 and upregulates its protein levels. The objectives of this phase I study were safety, pharmacokinetic profile, evaluation of the lymphocytes CD4+ and CD8+ and preliminary activity in patients with advanced tumors. A 3 + 3 dose-escalation design with seven dose levels was implemented.

Histological and transcriptomic analysis of adipose and muscle of dairy calves supplemented with 5-hydroxytryptophan.

In mammals, peripheral serotonin is involved in regulating energy balance. Herein, we characterized the transcriptomic profile and microstructure of adipose and muscle in pre-weaned calves with increased circulating serotonin. Holstein bull calves (21 ± 2 days old) were fed milk replacer supplemented with saline (CON, 8 mL/day n = 4) or 5-hydroxytryptophan (5-HTP, 90 mg/day, n = 4) for 10 consecutive days.

Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost .

Fish vary in their ability to biosynthesise long-chain polyunsaturated fatty acids (LC-PUFA) depending upon the complement and function of key enzymes commonly known as fatty acyl desaturases and elongases. It has been reported in the existence of a Δ4 desaturase, enabling the biosynthesis of docosahexaenoic acid (DHA) from eicosapentaenoic acid (EPA), which can be modulated by the diet. The present study aims to evaluate the combined effects of the partial replacement of fish oil (FO) with vegetable oils and reduced environmental salinity in the fatty acid composition of relevant body compartments (muscle, hepatocytes and enterocytes), the enzymatic activity over α-linolenic acid (ALA) to form n-3 LC-PUFA through the incubation of isolated hepatocytes and enterocytes with [1-C] 18:3 n-3, and the regulation of the and in the liver and intestine.

Endocrine Signals Altered by Heat Stress Impact Dairy Cow Mammary Cellular Processes at Different Stages of the Dry Period.

Hormonal alterations occurring under late gestation heat stress may disturb mammary gland remodelling, resulting in a reduced milk yield during the subsequent lactation. We investigated the effects of an altered endocrine environment on mammary gene expression at different stages of the dry period. Mammary gland biopsies from in vivo-cooled (CL) or heat-stressed (HT) cows were collected at d 3 and 35 relative to dry-off and divided into explants.

Photodynamic studies reveal rapid formation and appreciable turnover of tau inclusions.

Accumulation of the tau protein in fibrillar intracellular aggregates is a defining feature of multiple neurodegenerative diseases collectively referred to as tauopathies. Despite intensive study of tau, there is limited information on the formation and clearance dynamics of tau inclusions. Using rAAV vectors to mediate expression of Dendra2-tagged human wild-type, P301L and pro-aggregant P301L/S320F tau proteins, with and without the addition of exogenous tau fibrillar seeds, we evaluated tau inclusion dynamics in organotypic brain slice culture (BSC) models using long-term optical pulse labeling methodology.