Publications by authors named "Marlene Holder"
Article Synopsis
- * A new quantitative method for generating hydroxyl radicals is introduced, using common laboratory equipment and reagents to facilitate protein oxidative footprinting.
- * The effectiveness of this method is illustrated through oxidation analyses of various proteins, including lysozyme and RAS-monobody complexes, achieving high-resolution mapping of protein structures at the level of single amino acids.
Polycomb repressive complex 2 (PRC2) is a histone methyltransferase critical for maintaining gene silencing during eukaryotic development. In mammals, PRC2 activity is regulated in part by the selective incorporation of one of two paralogs of the catalytic subunit, EZH1 or EZH2. Each of these enzymes has specialized biological functions that may be partially explained by differences in the multivalent interactions they mediate with chromatin.
View Article and Find Full Text PDFThe maintenance of gene expression patterns during metazoan development is achieved, in part, by the actions of polycomb repressive complex 2 (PRC2). PRC2 catalyzes mono-, di-, and trimethylation of histone H3 at lysine 27 (H3K27), with H3K27me2/3 being strongly associated with silenced genes. We demonstrate that EZH1 and EZH2, the two mutually exclusive catalytic subunits of PRC2, are differentially activated by various mechanisms.
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