Targeting Transcriptional and Translational Hindrances in a Modular T7RNAP Expression System in Engineered .

The T7 RNA polymerase is considered one of the most popular tools for heterologous gene expression in the gold standard biotechnological host . However, the exploitation of this tool in other prospective hosts, such as the biotechnologically relevant bacterium , is still very scarce. The majority of the existing T7-based systems in show low expression strengths and possess only weak controllability.

Trehalose production by Cupriavidus necator from CO and hydrogen gas.

In this work, the hydrogen-oxidizing bacterium Cupriavidus necator H16 was engineered for trehalose production from gaseous substrates. First, it could be shown that C. necator is a natural producer of trehalose when stressed with sodium chloride.