Publications by authors named "Marlane G Seeger"
Multidrug-resistant (MDR) microorganisms pose a threat to animal health, particularly in integumentary diseases, which can be caused by multiple organisms and often manifest as biofilms, hindering treatment effectiveness. We evaluated the antimicrobial activity of antimicrobial photodynamic therapy (aPDT) using a water-soluble tetra-cationic porphyrin (4-HTMeP) against MDR bacteria cultured in biofilm and in mono and polyculture grown on canine skin samples. We utilized 4-HTMeP porphyrin against MDR Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus pseudintermedius.
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- Infectious bovine keratoconjunctivitis (IBK) is a major eye disease affecting cattle, caused primarily by the bacteria Moraxella bovis and Moraxella bovoculi, which can form biofilms.
- Traditional treatments with antimicrobials have limited effectiveness in clearing these infections, leading to exploration of alternative therapies like photodynamic therapy using porphyrins.
- A study found that a zinc(II) metalloporphyrin (ZnTMeP) reduced biofilm formation and partially destroyed existing biofilms of M. bovoculi, but did not affect M. bovis biofilms, indicating potential for further research into using phototherapy for treating IBK.
Multidrug-resistant (MDR) organisms have been frequently isolated from integumentary lesions of animals, and these lesions are usually infected by more than one pathogen. This study evaluated an in vitro antimicrobial photodynamic therapy (aPDT) using two water-soluble tetra-cationic porphyrins (3-HTMeP and 4-HTMeP) against mono and polyculture of MDR bacteria isolated from dogs, cats, and horses. Ten isolates of MDR bacteria (two of each species: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia marcescens, and Staphylococcus pseudointermedius) were used to evaluate aPDT against the monoculture using a non-cytotoxic concentration of 3-HTMeP and 4-HTMeP porphyrins (40 µM), with 30 min of light irradiation in Gram-positive and 90 min for Gram-negative bacteria.
View Article and Find Full Text PDFA 2-year-old female African pygmy hedgehog (Atelerix albiventris) with a cutaneous nodular lesion on the dorsal surface of the right forelimb was presented for clinical examination. Histopathological findings included granulomatous dermatitis with extensive necrosis. Long and slender acid-fast bacilli were seen within the cytoplasm of macrophages and in extracellular spaces.
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