Publications by authors named "Markus R Busch"
Article Synopsis
- The study explores how new enzymatic activities can rapidly evolve from existing ones, using the herbicide atrazine as a case study.
- Researchers focused on the enzyme AtzB, which breaks down atrazine, examining its side activities to uncover its evolutionary origins.
- The findings indicate that small mutations can greatly enhance enzyme activity, demonstrating how minimal changes can lead to significant evolutionary advancements in enzymatic function.
We present a detailed structure-function analysis of the ureidoacrylate amidohydrolase RutB from , which is an essential enzyme of the Rut pathway for pyrimidine utilization. Crystals of selenomethionine-labeled RutB were produced, which allowed us to determine the first structure of the enzyme at a resolution of 1.9 Å and to identify it as a new member of the isochorismatase-like hydrolase family.
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