Resonance enhanced dynamic light scattering.

We present a novel light scattering setup that enables probing of dynamics near solid surfaces. An evanescent wave generated by a surface plasmon resonance in a metal layer is the incident light field in the dynamic light scattering experiment. The combination of surface plasmon resonance spectroscopy and dynamic light scattering leads to a spatiotemporal resolution extending a few hundred nanometers from the surface and from microseconds to seconds.

Probing dynamics at interfaces: resonance enhanced dynamic light scattering.

Experiments addressing supramolecular dynamics at interfaces are of paramount importance for the understanding of the dynamic behaviour of polymers, particles, or cells at interfaces, transport phenomena to and from surfaces, thin films or membranes. However, there are only few reports in the literature due to the paucity of experimental methods that offer the required spatial and time resolution. Evanescent wave dynamic light scattering originally developed to meet these needs has limited sensitivity and is restricted to glass substrates.

Electron transfer kinetics of cytochrome c probed by time-resolved surface-enhanced resonance Raman spectroscopy.

An improved setup including a measuring cell was designed for time-resolved surface-enhanced resonance Raman (SERR) spectroscopy. The cell is based on a rotating disk electrode (RDE) made from electrochemically roughened Ag. Cytochrome c (cc) adsorbed on a monolayer of mercaptoethanol is investigated with respect to heterogeneous electron transfer.

In situ monitoring of the catalytic activity of cytochrome C oxidase in a biomimetic architecture.

Cytochrome c oxidase (CcO) from Paracoccus denitrificans was immobilized in a strict orientation via a his-tag attached to subunit I on a gold film and reconstituted in situ into a protein-tethered bilayer lipid membrane. In this orientation, the cytochrome c (cyt c) binding site is directed away from the electrode pointing to the outer side of the protein-tethered bilayer lipid membrane architecture. The CcO can thus be activated by cyt c under aerobic conditions.