Publications by authors named "Marko Blazic"

Article Synopsis
  • The study explores the role of crotonyl-CoA carboxylase/reductase (Ccr) homologues in S. tsukubaensis, particularly focusing on two identified homologues: ccr1 and allR, and their contributions to the biosynthesis of FK506 and related compounds.
  • While ccr1 shows limited involvement in FK506 or FK520 biosynthesis under normal conditions, it can contribute to ethylmalonyl-CoA production when overexpressed.
  • Inactivation of ccr1 disrupts the EMC pathway, hindering growth on acetate, which emphasizes the intricate connections between primary and secondary metabolic pathways in this microorganism.
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Streptomyces rapamycinicus strain NRRL 5491 produces the important drug rapamycin. It has a large genome of 12.7 Mb, of which over 3 Mb consists of 48 secondary metabolite biosynthesis clusters.

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Background: FK506 (Tacrolimus) is an important immunosuppressant, produced by industrial biosynthetic processes using various Streptomyces species. Considering the complex structure of FK506, it is reasonable to expect complex regulatory networks controlling its biosynthesis. Regulatory elements, present in gene clusters can have a profound influence on the final yield of target product and can play an important role in development of industrial bioprocesses.

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The high G+C content and large genome size make the sequencing and assembly of Streptomyces genomes more difficult than for other bacteria. Many pharmaceutically important natural products are synthesized by modular polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs). The analysis of such gene clusters is difficult if the genome sequence is not of the highest quality, because clusters can be distributed over several contigs, and sequencing errors can introduce apparent frameshifts into the large PKS and NRPS proteins.

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