Publications by authors named "Mark Wefers Bettink"
Article Synopsis
- The study introduces the Cellular Oxygen METabolism (COMET®) monitor, which measures mitochondrial oxygen tension (mitoPO2) using a specific technique, aiming to assess its effectiveness in surgical environments.
- In observing 20 neurosurgical patients, researchers measured mitoPO2 alongside other vital parameters like tissue oxygenation and blood flow, finding that all patients remained stable throughout the procedures.
- Results indicated a baseline mitoPO2 of 60 mmHg, with levels generally stable but decreasing over time; this establishes the COMET® monitor as a reliable tool for future research on mitochondrial oxygenation in surgery.
Article Synopsis
- The study investigates the feasibility of measuring skin mitochondrial partial oxygen pressure (mitoPO) during cardiac arrest and extracorporeal cardiopulmonary resuscitation (ECPR) in pigs.
- It found that continuous mitoPO measurements are possible, with results showing a delay in initial mitoPO spikes after ECPR initiation, and that mitoPO did not consistently correlate with mean arterial pressure (MAP).
- The findings suggest the need for alternative metrics beyond MAP to better assess tissue perfusion quality during cardiac arrest and ECPR, with one pig surviving the experimental conditions.
Current monitoring techniques in neonates lack sensitivity for hypoxia at cellular level. The recent introduction of the non-invasive Cellular Oxygen METabolism (COMET) monitor enables measuring mitochondrial oxygen tension (mitoPO), based on oxygen-dependent quenching of delayed fluorescence of 5-aminolevulinic acid (ALA)-enhanced protoporphyrin IX. The aim is to determine the feasibility and safety of non-invasive mitoPO monitoring in surgical newborns.
View Article and Find Full Text PDFMitochondrial function has been predominantly measured ex vivo. Due to isolation and preservation procedures ex vivo measurements might misrepresent in vivo mitochondrial conditions. Direct measurement of in vivo mitochondrial oxygen tension (mitoPO) and oxygen disappearance rate (ODR) with the protoporphyrin IX-triplet state lifetime technique (PpIX-TSLT) might increase our understanding of mitochondrial dysfunction in the pathophysiology of acute disease.
View Article and Find Full Text PDFBackground: We determined the prevalence and clinical consequences of herpes simplex virus (HSV) type 1 (HSV-1), HSV type 2 (HSV-2), and varicella-zoster virus (VZV) in cornea tissues obtained after penetrating keratoplasty (PKP) was performed.
Methods: The excised corneas of 83 patients with a history of herpetic keratitis (HK; hereafter referred to as "patients with HK") and 367 patients without a history of HK (hereafter referred to "patients without HK") were analyzed by real-time polymerase chain reaction (PCR) and virus culture for the presence of HSV-1, HSV-2, and VZV. In addition, 273 post-PKP donor corneoscleral rims were analyzed.