Application of whole genome re-sequencing data in the development of diagnostic DNA markers tightly linked to a disease-resistance locus for marker-assisted selection in lupin (Lupinus angustifolius).

Background: Molecular marker-assisted breeding provides an efficient tool to develop improved crop varieties. A major challenge for the broad application of markers in marker-assisted selection is that the marker phenotypes must match plant phenotypes in a wide range of breeding germplasm. In this study, we used the legume crop species Lupinus angustifolius (lupin) to demonstrate the utility of whole genome sequencing and re-sequencing on the development of diagnostic markers for molecular plant breeding.

Draft genome sequence, and a sequence-defined genetic linkage map of the legume crop species Lupinus angustifolius L.

Lupin (Lupinus angustifolius L.) is the most recently domesticated crop in major agricultural cultivation. Its seeds are high in protein and dietary fibre, but low in oil and starch.

Rapid development of molecular markers by next-generation sequencing linked to a gene conferring phomopsis stem blight disease resistance for marker-assisted selection in lupin (Lupinus angustifolius L.) breeding.

Selection for phomopsis stem blight disease (PSB) resistance is one of the key objectives in lupin (Lupinus angustifolius L.) breeding programs. A cross was made between cultivar Tanjil (resistant to PSB) and Unicrop (susceptible).

Application of next-generation sequencing for rapid marker development in molecular plant breeding: a case study on anthracnose disease resistance in Lupinus angustifolius L.

Background: In the last 30 years, a number of DNA fingerprinting methods such as RFLP, RAPD, AFLP, SSR, DArT, have been extensively used in marker development for molecular plant breeding. However, it remains a daunting task to identify highly polymorphic and closely linked molecular markers for a target trait for molecular marker-assisted selection. The next-generation sequencing (NGS) technology is far more powerful than any existing generic DNA fingerprinting methods in generating DNA markers.

A PCR-based molecular marker applicable for marker-assisted selection for anthracnose disease resistance in lupin breeding.

Selection for anthracnose disease resistance is one of the major objectives in lupin breeding programs. The aim of this study was to develop a molecular marker linked to a gene conferring anthracnose resistance in narrow-leafed lupin (Lupinus angustifolius L.), which can be widely used for MAS in lupin breeding.

Characterization of Colletotrichum gloeosporioides Isolates from Ornamental Lupines in Connecticut.

Twenty-six isolates of Colletotrichum gloeosporioides were isolated from diseased ornamental lupines (Lupinus spp. 'Russell Hybrids') in seven different nurseries in Connecticut from 1996 to 1998. Three isolates from New Hampshire, New York, and Utah were also included.

Characterization and Pathogenicity of Rhizoctonia Species on Canola.

A total of 112 Rhizoctonia isolates were collected from various canola (Brassica napus) growing areas of Western Australia. Pectic enzyme electrophoresis differentiated these isolates into six distinct zymogram groups: R. solani, 54% ZG5 (AG2-1), 8% ZG6 (AG2-1), and 1% ZG9 (AG10); binucleate Rhizoctonia, 12% CZG1 (CAG1), 4% CZG4, and 6% CZG5 (AGK); and the remainder unidentified binucleate groups (15%).