Publications by authors named "Mark Trulson"
The ability to precisely monitor and manipulate neural circuits is essential to understand the brain. Advancements over the last decade in optical techniques such as calcium imaging and optogenetics have empowered researchers to gain insight into brain function by systematically manipulating or monitoring defined neural circuits. Combining these cutting-edge techniques enables a more direct mechanism for ascribing neural dynamics to behavior.
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- The study showcases a cutting-edge technique for sequencing DNA at the single-molecule level, using a DNA polymerase and four unique fluorescently labeled dNTPs for real-time monitoring.
- By employing zero-mode waveguide nanostructures, researchers achieved simultaneous detection of thousands of sequencing reactions while minimizing interference during the observation of DNA synthesis.
- Results demonstrated high accuracy in sequencing, with a 99.3% median accuracy and detailed insights into polymerase behavior, including different polymerization states influenced by the DNA’s secondary structure.
We have investigated the kinetics of DNA hybridization to oligonucleotide arrays on high-capacity porous silica films that were deposited by two techniques. Films created by spin coating pure colloidal silica suspensions onto a substrate had pores of approximately 23 nm, relatively low porosity (35%), and a surface area of 17 times flat glass (for a 0.3-microm film).
View Article and Find Full Text PDFThe hybridization kinetics of oligonucleotide targets to oligonucleotide probe arrays synthesized using photolithographic fabrication methods developed by Affymetrix have been measured. Values for the fundamental adsorption parameters, k(a), k(d), and K, were determined at both room temperature and 45 degrees C by monitoring the hybridization of fluorescently labeled targets to the array. The values for these parameters and the adsorbed target density (