Cryopreservation of Populus trichocarpa and Salix dormant buds with recovery by grafting or direct rooting.

Background: Methods are needed for the conservation of clonally maintained trees of Populus and Salix. In this work, Populus trichocarpa and Salix genetic resources were cryopreserved using dormant scions as the source explant.

Objective: We quantified the recovery of cryopreserved materials that originated from diverse field environments by using either direct sprouting or grafting.