Publications by authors named "Mark Kutschke"
Article Synopsis
- Mass spectrometry (MS) is a powerful tool for detecting chemical species but has limitations in processing speed, which can be improved by integrating it with microfluidics.
- The Drop-NIMS platform combines a microfluidic device with a unique MS technique that allows for the random mixing of droplets to create a library of enzymatic reactions directly on the NIMS surface, minimizing handling.
- This method was successfully used to quickly screen low-volume enzymatic reactions, identifying various glycoside hydrolases relevant to industries like food and biofuels, demonstrating its versatility and ease of use for analyzing small molecule metabolites.
Enzymatic deconstruction of lignocellulosic biomass is crucial to establishment of the renewable biofuel and bioproduct economy. Better understanding of these enzymes, including their catalytic and binding domains, and other features offer potential avenues for improvement. Glycoside hydrolase family 9 (GH9) enzymes are attractive targets because they have members that exhibit exo- and endo-cellulolytic activity, processivity of reaction, and thermostability.
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