Publications by authors named "Mark F Foecking"

Presented here is the first completely assembled genome sequence of Kocuria palustris, an actinobacterial species with broad ecological distribution. The single, circular chromosome of K. palustris MU14/1 comprises 2,854,447 bp, has a G+C content of 70.

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  • - The genome sequence of a new Mycoplasma strain, named Mycoplasma sp. HU2014, has been sequenced, consisting of 1,084,927 nucleotides.
  • - This strain was isolated from mycoplasma-infected chicken DT40 cells.
  • - Phylogenetic analysis indicates that Mycoplasma sp. HU2014 is closely related to Mycoplasma yeatsii and Mycoplasma cottewii.
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Presented here is the complete and annotated genome sequence of Mycoplasma hominis Sprott (ATCC 33131). The chromosome comprises 695,214 bp, which is approximately 30 kb larger than the syntenic genome of M. hominis PG21(T).

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Mycoplasma capricolum subspecies capricolum is both a pathogen of small ruminants and a model recipient organism for gene transplantation and synthetic biology. With the availability of the complete genome of the type strain California kid (released in 2005), a draft genome of strain GM508D was determined to investigate genomic variation in this subspecies. Differences in mobile genetic element location and complement, catabolic pathway genes, contingency loci, surface antigen genes and type II restriction-modification systems highlight the plasticity and diversity within this taxon.

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Mycoplasma yeatsii is a goat mycoplasma species that, although an obligate parasite, accommodates this lifestyle as an inapparent commensalist. High-frequency transformation has also been reported for this species. The complete 895,051-bp genome sequence of strain GM274B has been determined, enabling an analysis of the features of this potential cloning host.

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Mycoplasma flocculare is a commensal or low-virulence pathogen of swine. The complete 778,866-bp genome sequence of M. flocculare strain Ms42(T) has been determined, enabling further comparison to genomes of the closely related pathogen Mycoplasma hyopneumoniae.

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The complete genome sequence of Mycoplasma hominis LBD-4 has been determined and the gene content ascribed. The 715,165-bp chromosome contains 620 genes, including 14 carried by a strain-variable prophage genome related to Mycoplasma fermentans MFV-1 and Mycoplasma arthritidis MAV-1. Comparative analysis with the genome of M.

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  • Staphylococcus hyicus causes exudative epidermitis, a skin disease in pigs.
  • A complete genome analysis of its type strain showed it has a type VII secretion system.
  • The genome also contains a large chromosomal island with genes for exfoliative toxin ExhA and a related EDIN toxin.
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  • The genome of Actinobacillus suis ATCC 33415(T) is 2,501,598 base pairs long and contains 2,246 open reading frames (ORFs).
  • A unique feature of this strain is the presence of an integrative conjugative element that has integrated into a tRNA locus.
  • Analyzing the gene set will enhance our knowledge of the bacteria's disease-causing mechanisms, genetic adaptability, and differences between serotypes.
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Presented here is a draft genome sequence for Staphylococcus agnetis CBMRN 20813338, isolated from a lactating dairy cow with subclinical mastitis. The genome is approximately 2,416 kb and has 35.79% G+C content.

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  • Coagulase-negative staphylococci, especially Staphylococcus chromogenes, frequently cause subclinical mastitis in cows.
  • The draft genome sequence of S. chromogenes strain MU 970 was obtained from milk of a cow with a long-term intramammary infection.
  • This research contributes valuable genomic data to understand the pathogenicity and treatment of mastitis caused by this bacterial species.
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Mycoplasma californicum is one of several mycoplasmal species associated with bovine mastitis. The complete genome sequence of 793,841 bp has been determined and annotated for the M. californicum ST-6 type strain, providing a resource for the identification of surface antigens and putative pathoadaptive features.

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Moraxella bovoculi is a recently identified species, recovered from the bovine eye, which is under investigation as an etiological agent of infectious bovine keratoconjunctivitis. A draft genome sequence of the Moraxella bovoculi type strain 237(T) has been determined to identify features that may be important during host colonization.

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Fusobacterium necrophorum is a Gram-negative anaerobic bacterium that causes foot rot and liver abscesses in cattle. F. necrophorum subsp.

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Bovine ocular infections compromise animal health and result in significant economic losses. Mycoplasma bovoculi is an etiological agent of conjunctivitis. Presented here is the 760,240-bp complete genome sequence of the M.

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Coagulase-negative staphylococci are frequently isolated from cases of subclinical bovine mastitis. Reported here is a draft genome sequence of Staphylococcus simulans UMC-CNS-990, an isolate recovered from a chronic intramammary infection of a Holstein cow. Unexpectedly, a cluster of genes encoding gas vesicle proteins was found within the 2,755-kb genome.

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Intramammary infections in dairy cattle are frequently caused by staphylococci, resulting in mastitis and associated economic losses. A draft genome sequence was determined for Staphylococcus equorum UMC-CNS-924, isolated from the milk of a Holstein cow, to better understand the genetic basis of its pathogenesis and adaptation to the bovine mammary gland.

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A circular plasmid, pMyBK1, was detected in Mycoplasma yeatsii strain GIH(T). Analysis of the sequence of the 3432-bp replicon identified two predicted open reading frames (ORFs), one with sequence similarity to multiple plasmid mobilization proteins and one that matches only to hypothetical ORFs encoded by integrated chromosomal elements in the sequenced genomes of two Mycoplasma species. Shuttle vectors were constructed in Escherichia coli which could be introduced into M.

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  • Mycoplasma mycoides subsp. mycoides small colony biotype (SC) is a serious animal pathogen responsible for contagious bovine pleuropneumonia.
  • The complete genome sequences of the pathogenic strain M. mycoides subsp. mycoides SC Gladysdale and a related pathogen, Mycoplasma leachii PG50(T), have been reported.
  • These studies enhance our understanding of the genetic makeup of these harmful bacteria within the same phylogenetic group affecting cattle.
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Mycoplasma hyorhinis impacts swine health and production in many countries, either as a primary pathogen or as a component of a polymicrobial infection. Isolates of this species are also common contaminants of tissue culture lines. The genome sequence of the cell culture isolate M.

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Mycoplasma putrefaciens is a causative agent of contagious agalactia in goats. Reported herein is the complete genome sequence of the M. putrefaciens type strain KS1.

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This complete and fully assembled genome sequence of Mycoplasma bovis type strain PG45 is the first available for this species and offers a framework for comparison with additional pathogenic isolates. The single circular chromosome of 1,003,404 bp reveals multiple gene sets and mechanisms involved in variable expression of surface antigens and the incursion of numerous and assorted mobile elements, despite its reduced size.

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Background: Intragenic tandem repeats occur throughout all domains of life and impart functional and structural variability to diverse translation products. Repeat proteins confer distinctive surface phenotypes to many unicellular organisms, including those with minimal genomes such as the wall-less bacterial monoderms, Mollicutes. One such repeat pattern in this clade is distributed in a manner suggesting its exchange by horizontal gene transfer (HGT).

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  • - The study of Mollicutes (mycoplasmas) shows that surface variation among different species is caused by random expression of various lipoprotein genes, crucial for their adaptability and survival as wall-less organisms.
  • - Research focused on two specific mycoplasma species revealed six novel lipoprotein genes (vmcA to vmcF) that contribute to a unique surface architecture, with two distinct gene locations influencing their expression patterns.
  • - These findings suggest that the variable lipoprotein genes not only highlight differences in mycoplasma pathogens' adaptability but also present potential targets for diagnostics in veterinary medicine.
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