Comparative analysis of cherry virus A genome sequences assembled from deep sequencing data.

Cherry virus A (CVA) is a ubiquitous graft-transmissible virus that mainly infects Prunus spp. Next-generation sequencing was applied to 39 tree fruit specimens infected with CVA, and 75 full and 16 partial-length CVA genome sequences were assembled. Phylogenetic analysis of these and 11 previously sequenced CVA genomes resulted in six major clusters with no observable relationship between the host and the assembled genome sequences.

Screening for plant viruses by next generation sequencing using a modified double strand RNA extraction protocol with an internal amplification control.

The majority of plant viruses contain RNA genomes. Detection of viral RNA genomes in infected plant material by next generation sequencing (NGS) is possible through the extraction and sequencing of total RNA, total RNA devoid of ribosomal RNA, small RNA interference (RNAi) molecules, or double stranded RNA (dsRNA). Plants do not typically produce high molecular weight dsRNA, therefore the presence of dsRNA makes it an attractive target for plant virus diagnostics.

Occurrence and Detection of Globodera rostochiensis on Vancouver Island, British Columbia: An Update.

The potato cyst nematode (PCN), Globodera rostochiensis, has been present in Central Saanich on Vancouver Island for at least 45 years. Eradication/control efforts have been ongoing, with regulations enacted in the early 1980s restricting the planting of host crops and movement of soil. Surveys monitoring for cyst populations have been minimal since the regulations have been in place with only one limited study in the early 1990s.

Detection of genetically modified canola using multiplex PCR coupled with oligonucleotide microarray hybridization.

A rapid method was developed for concurrent screening of transgenic elements in GM canola. This method utilizes a single multiplex PCR coupled with an oligonucleotide DNA array capable of simultaneously detecting the 12 approved GM canola lines in Canada. The assay includes construct-specific elements for identification of approved lines, common elements (e.