Maladaptive positive feedback production of ChREBPβ underlies glucotoxic β-cell failure.

Preservation and expansion of β-cell mass is a therapeutic goal for diabetes. Here we show that the hyperactive isoform of carbohydrate response-element binding protein (ChREBPβ) is a nuclear effector of hyperglycemic stress occurring in β-cells in response to prolonged glucose exposure, high-fat diet, and diabetes. We show that transient positive feedback induction of ChREBPβ is necessary for adaptive β-cell expansion in response to metabolic challenges.

Simultaneous and accurate measurement of the dielectric constant at many frequencies spanning a wide range.

We present an innovative technique which allows the simultaneous measurement of the dielectric constant of a material at many frequencies, spanning a four orders of magnitude range chosen between 10(-2) Hz and 10(4) Hz. The sensitivity and accuracy are comparable to those obtained using standard single frequency techniques. The technique is based on three new and simple features: (a) the precise real time correction of the amplification of a current amplifier, (b) the specific shape of the excitation signal and its frequency spectrum, and (c) the precise synchronization between the generation of the excitation signal and the acquisition of the dielectric response signal.