Continous, non-invasive monitoring of oxygen consumption in a parallelized microfluidic system provides novel insight into the response to nutrients and drugs of primary human hepatocytes.

Oxygen plays a fundamental role in cellular energy metabolism, differentiation and cell biology in general. Consequently, oxygen sensing can be used to assess cell vitality and detect specific mechanisms of toxicity. In 2D models currently used, the oxygen supply provided by diffusion is generally too low, especially for cells having a high oxygen demand.

Parallelizable Microfluidic Platform to Model and Assess In Vitro Cellular Barriers: Technology and Application to Study the Interaction of 3D Tumor Spheroids with Cellular Barriers.

Endothelial and epithelial cellular barriers play a vital role in the selective transport of solutes and other molecules. The properties and function of these barriers are often affected in case of inflammation and disease. Modelling cellular barriers in vitro can greatly facilitate studies of inflammation, disease mechanisms and progression, and in addition, can be exploited for drug screening and discovery.

HepaChip-MP - a twenty-four chamber microplate for a continuously perfused liver coculture model.

HepaChip microplate (HepaChip-MP) is a microfluidic platform comprised of 24 independent culture chambers with continuous, unidirectional perfusion. In the HepaChip-MP, an automated dielectrophoresis process selectively assembles viable cells into elongated micro tissues. Freshly isolated primary human hepatocytes (PHH) and primary human liver endothelial cells (HuLEC) were successfully assembled as cocultures aiming to mimic the liver sinusoid.