Impact of nanosilver on various DNA lesions and HPRT gene mutations - effects of charge and surface coating.

Background: The main goal of this research was to study the interactions of a fully characterized set of silver nanomaterials (Ag ENMs) with cells in vitro, according to the standards of Good Laboratory Practices (GLP), to assure the quality of nanotoxicology research. We were interested in whether Ag ENMs synthesized by the same method, with the same size distribution, shape and specific surface area, but with different charges and surface compositions could give different biological responses.

Methods: A range of methods and toxicity endpoints were applied to study the impacts of interaction of the Ag ENMs with TK6 cells.