Publications by authors named "Marissa Danielle Acciani"
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the most recent global pandemic that has caused more than a million deaths around the world. The spike glycoprotein (S) drives the entry and fusion of this virus and is the main determinant of cell tropism. To explore S requirements for entry under BSL2 conditions, S has been pseudotyped onto vesicular stomatitis virus (VSV) or retroviral particles with varied success.
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- Viral entry is the initial step in the replication cycle of enveloped viruses, facilitated by specific viral glycoproteins with varying receptor affinities.
- The study utilized vesicular stomatitis virus (VSV) to assess the entry efficiencies of different glycoproteins, including those from SARS-CoV-2, Ebola, Lassa, and Chikungunya, by creating recombinant VSV viruses that express these proteins.
- Utilizing a reporter gene and live-cell substrates, the research revealed that glycoproteins needing more priming generally resulted in slower entry times, while also enabling the analysis of receptor preferences and endocytosis mechanisms for a better understanding of viral entry.